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Literature summary for 2.4.1.25 extracted from

  • Rudeekulthamrong, P.; Kaulpiboon, J.
    Optimization of amylomaltase for the synthesis of alpha-arbutin derivatives as tyrosinase inhibitors (2020), Carbohydr. Res., 494, 108078.
    View publication on PubMed
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Application

Application Comment Organism
synthesis synthesis of alpha-arbutin derivatives as tyrosinase (EC 1.14.18.1) inhibitors by engineered amylomaltase, production features comparison with cyclomaltodextrin glucanotransferase (CGTase, 2.4.1.19), overview. Incubation of AMase with the tapioca starch donor and an alpha- or beta-arbutin acceptor yields at least three glycoside products. AMase has higher disproportionation and cyclization activities but lower hydrolysis activity compared to CGTase Therminus sp.

Protein Variants

Protein Variants Comment Organism
additional information optimization of amylomaltase for the synthesis of alpha-arbutin derivatives as tyrosinase inhibitors. Incubation of AMase with the tapioca starch donor and an alpha- or beta-arbutin acceptor yields at least three glycoside products. AMase has higher disproportionation and cyclization activities but lower hydrolysis activity compared to CGTase Therminus sp.

Organism

Organism UniProt Comment Textmining
Therminus sp.
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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 Therminus sp.
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information alpha- and beta-arbutin-alpha-D-glycosides are formed through transglycosylation reactions catalyzed by a recombinant amylomaltase, using tapioca starch and alpha- and beta-arbutin as donor and acceptor molecules, respectively. The glycoside products are isolated and analyzed by HPLC, and two major products are identified by mass spectrometry and nuclear magnetic resonance spectroscopy, namely, alpha-arbutin-alpha-D-glucopyranoside (alpha-Ab-alpha-G1) and alpha-arbutin-alpha-D-maltopyranoside (alpha-Ab-alpha-G2). Both alpha-Ab-alpha-G1 and alpha-Ab-alpha-G2 are more water soluble than alpha-arbutin Therminus sp. ?
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Synonyms

Synonyms Comment Organism
AMase
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 Therminus sp.
amylomaltase
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 Therminus sp.