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Literature summary for 2.4.1.25 extracted from

  • Nimpiboon, P.; Tumhom, S.; Nakapong, S.; Pongsawasdi, P.
    Amylomaltase from Thermus filiformis expression in Saccharomyces cerevisiae and its use in starch modification (2020), J. Appl. Microbiol., 129, 1287-1296.
    View publication on PubMed
Search for more literature for 2.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain FGY217 Thermus filiformis

Organism

Organism UniProt Comment Textmining
Thermus filiformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap affinity column chromatography Thermus filiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cassava starch + glycosyl acceptor
-
 Thermus filiformis large-ring cyclodextrins 24-29
-
 ?
pea starch + glycosyl acceptor
-
 Thermus filiformis large-ring cyclodextrins 24-29
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Thermus filiformis

Synonyms

Synonyms Comment Organism
4alphaGTase
-
 Thermus filiformis
amylomaltase
-
 Thermus filiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermus filiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the thermostability at 60-70°C in pH 6.0 potassium phosphate buffer, after 2 h of incubation time, shows approximately 70-80% remaining activity Thermus filiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Thermus filiformis