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Literature summary for 2.4.1.25 extracted from

  • Tumhom, S.; Krusong, K.; Pongsawasdi, P.
    Y418 in 410s loop is required for high transglucosylation activity and large-ring cyclodextrin production of amylomaltase from Corynebacterium glutamicum (2017), Biochem. Biophys. Res. Commun., 488, 516-521 .
    View publication on PubMed
Search for more literature for 2.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
Y418A the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
Y418D the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
Y418F the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 29-33 from pea starch Corynebacterium glutamicum
Y418R the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
Y418S the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
Y418W the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15.7
-
 maltotriose mutant enzyme Y418A, at pH 6.0 and 30°C Corynebacterium glutamicum
16.3
-
 maltotriose mutant enzyme Y418D, at pH 6.0 and 30°C Corynebacterium glutamicum
17.2
-
 maltotriose wild type enzyme, at pH 6.0 and 30°C Corynebacterium glutamicum
18
-
 maltotriose mutant enzyme Y418S, at pH 6.0 and 30°C Corynebacterium glutamicum
18.2
-
 maltotriose mutant enzyme Y418F, at pH 6.0 and 30°C Corynebacterium glutamicum
19.5
-
 maltotriose mutant enzyme Y418R, at pH 6.0 and 30°C Corynebacterium glutamicum
20.4
-
 maltotriose mutant enzyme Y418W, at pH 6.0 and 30°C Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + glycosyl acceptor worst substrate Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
maltohexaose + glycosyl acceptor
-
 Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
maltopentaose + glycosyl acceptor
-
 Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
maltotetraose + glycosyl acceptor second best substrate Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
maltotriose + glycosyl acceptor best substrate Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
pea starch + glycosyl acceptor
-
 Corynebacterium glutamicum large ring-cyclodextrins 29-33
-
 ?

Subunits

Subunits Comment Organism
? x * 84000, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
amylomaltase
-
 Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
258
-
 maltotriose mutant enzyme Y418R, at pH 6.0 and 30°C Corynebacterium glutamicum
403
-
 maltotriose mutant enzyme Y418W, at pH 6.0 and 30°C Corynebacterium glutamicum
1833
-
 maltotriose mutant enzyme Y418S, at pH 6.0 and 30°C Corynebacterium glutamicum
1867
-
 maltotriose mutant enzyme Y418D, at pH 6.0 and 30°C Corynebacterium glutamicum
1967
-
 maltotriose mutant enzyme Y418A, at pH 6.0 and 30°C Corynebacterium glutamicum
2667
-
 maltotriose mutant enzyme Y418F, at pH 6.0 and 30°C Corynebacterium glutamicum
2800
-
 maltotriose wild type enzyme, at pH 6.0 and 30°C Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.3
-
 maltotriose mutant enzyme Y418R, at pH 6.0 and 30°C Corynebacterium glutamicum
19.7
-
 maltotriose mutant enzyme Y418W, at pH 6.0 and 30°C Corynebacterium glutamicum
101.8
-
 maltotriose mutant enzyme Y418S, at pH 6.0 and 30°C Corynebacterium glutamicum
115
-
 maltotriose mutant enzyme Y418D, at pH 6.0 and 30°C Corynebacterium glutamicum
125.3
-
 maltotriose mutant enzyme Y418A, at pH 6.0 and 30°C Corynebacterium glutamicum
146.7
-
 maltotriose mutant enzyme Y418F, at pH 6.0 and 30°C Corynebacterium glutamicum
163.3
-
 maltotriose wild type enzyme, at pH 6.0 and 30°C Corynebacterium glutamicum