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Literature summary for 2.4.1.25 extracted from
- Maltose metabolism in the hyperthermophilic archaeon Thermococcus litoralis Purification and characterization of key enzymes (1999), J. Bacteriol., 181, 3358-3367 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermococcus litoralis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
maltoheptaose | pH 7.0, 95°C | Thermococcus litoralis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 134000 | - |
gel filtration | Thermococcus litoralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus litoralis | O32462 | DSM 5473 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus litoralis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.7 | - |
substrate: maltotriose, pH 7.0, 95°C | Thermococcus litoralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltoheptaose | - |
Thermococcus litoralis | maltodextrin + D-glucose | - |
? | |
| maltose + D-glucose | - |
Thermococcus litoralis | maltodextrin + D-glucose | - |
? | |
| maltotriose | - |
Thermococcus litoralis | maltodextrin + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 79000, SDS-PAGE | Thermococcus litoralis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-alpha-glucanotransferase | - |
Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Thermococcus litoralis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 105 | 70°C: about 60% of maximal activity, 105°C: about 50% of maximal activity | Thermococcus litoralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Thermococcus litoralis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermococcus litoralis | isoelectric focusing | - |
3.5 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Thermococcus litoralis | the enzyme is constitutive | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | key enzymes in the pathway for maltose catabolism. Glucose derived from the action of 4-alpha-glucanotransferase is subsequently metabolized via an Embden-Meyerhof pathway | Thermococcus litoralis |
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