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Literature summary for 2.4.1.25 extracted from

  • Tantanarat, K.; ONeill, E.; Rejzek, M.; Field, R.; Limpaseni, T.
    Expression and characterization of 4-alpha-glucanotransferase genes from Manihot esculenta Crantz and Arabidopsis thaliana and their use for the production of cycloamyloses (2014), Process Biochem., 49, 84-89.
No PubMed abstract available
Search for more literature for 2.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) One Shot cells Arabidopsis thaliana
expressed in Escherichia coli BL21 Star (DE3) One Shot cells Manihot esculenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
27.5
-
 maltotriose at pH 7.0 and 37 °C Arabidopsis thaliana
69.2
-
 maltotriose at pH 7.0 and 37 °C Manihot esculenta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59400
-
 2 * 59400, calculated from amino acid sequence Manihot esculenta
66000
-
 2 * 66000, SDS-PAGE Arabidopsis thaliana
117000
-
 gel filtration Manihot esculenta
123000
-
 gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LV91
-
-
Manihot esculenta
-
 cultivar KU50
-

Purification (Commentary)

Purification (Comment) Organism
Ni HiTrap column chromatography and Superdex 75 gel filtration Arabidopsis thaliana
Ni HiTrap column chromatography and Superdex 75 gel filtration Manihot esculenta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltotriose + maltotriose
-
 Manihot esculenta maltooligosaccharides
-
 ?
maltotriose + maltotriose
-
 Arabidopsis thaliana maltooligosaccharides
-
 ?
potato amylose + glycosyl acceptor
-
 Manihot esculenta cycloamylose the enzyme can produce cycloamyloses in the range 16 to more than 60 glucose residues ?
potato amylose + glycosyl acceptor
-
 Arabidopsis thaliana cycloamylose the enzyme can produce cycloamyloses in the range 16–50 glucose residues ?

Subunits

Subunits Comment Organism
homodimer 2 * 59400, calculated from amino acid sequence Manihot esculenta
homodimer 2 * 66000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
4alphaGTase
-
 Manihot esculenta
4alphaGTase
-
 Arabidopsis thaliana
DPE1
-
 Manihot esculenta
DPE1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Arabidopsis thaliana
37
-
-
 Manihot esculenta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
580
-
 maltotriose at pH 7.0 and 37 °C Arabidopsis thaliana
894
-
 maltotriose at pH 7.0 and 37 °C Manihot esculenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Manihot esculenta
6 8
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Manihot esculenta calculated from amino acid sequence
-
 5.1
Arabidopsis thaliana calculated from amino acid sequence
-
 5.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.9
-
 maltotriose at pH 7.0 and 37 °C Manihot esculenta
21.6
-
 maltotriose at pH 7.0 and 37 °C Arabidopsis thaliana