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Literature summary for 2.4.1.25 extracted from

  • Jung, J.; Jung, T.; Seo, D.; Yoon, S.; Choi, H.; Park, B.; Park, C.; Woo, E.
    Structural and functional analysis of substrate recognition by the 250s loop in amylomaltase from Thermus brockianus (2011), Proteins, 79, 633-644.
    View publication on PubMed
Search for more literature for 2.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus brockianus

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.3 A resolution. Structure shows a pattern of conformational flexibility in the 250s loop with higher B-factor. The conformational flexibility of the loop may be involved in substrate binding in the GH77 family Thermus brockianus

Protein Variants

Protein Variants Comment Organism
F251G mutation results in significantly lower glucose production but increased maltose production from maltopentose substrates, showing an altered substrate-binding affinity Thermus brockianus
Q256G mutation results in increased Km for maltotriose and a sharp decrease of the transglycosylation factor for maltose Thermus brockianus
W258G mutant shows neither cyclization nor coupling activity, suggesting that residue Trp258 plays an essential role in all catalytic activities including hydrolysis and transglycosylation activities Thermus brockianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 maltotriose wild-type, pH 6.0, 70°C Thermus brockianus
5.8
-
 maltotriose mutant F251G, pH 6.0, 70°C Thermus brockianus
9.5
-
 maltotriose mutant W258G, pH 6.0, 70°C Thermus brockianus
165
-
 maltotriose mutant Q256G, pH 6.0, 70°C Thermus brockianus

Organism

Organism UniProt Comment Textmining
Thermus brockianus Q2VJA0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltotriose
-
 Thermus brockianus maltooligosaccharides
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
 maltotriose mutant F251G, pH 6.0, 70°C Thermus brockianus
0.005
-
 maltotriose mutant W258G, pH 6.0, 70°C Thermus brockianus
0.015
-
 maltotriose wild-type, pH 6.0, 70°C Thermus brockianus
0.08
-
 maltotriose mutant Q256G, pH 6.0, 70°C Thermus brockianus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00035
-
 maltotriose mutant F251G, pH 6.0, 70°C Thermus brockianus
0.00048
-
 maltotriose mutant Q256G, pH 6.0, 70°C Thermus brockianus
0.00048
-
 maltotriose mutant W258G, pH 6.0, 70°C Thermus brockianus
0.007
-
 maltotriose wild-type, pH 6.0, 70°C Thermus brockianus