Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
Y172A | mutant exhibits lower disproportionation, cyclization, and hydrolysis activities than the wild-type. The kcat/Km of the disproportionation reaction for the Y172A enzyme is 2.8fold lower than that of wild-type. The Y172A enzyme shows a product pattern different from that of wild-type at a long incubation time. The principal large-ring cyclodextrin products of the Y172A mutant are a cycloamylose mixture with a degree of polymerization of 28 or 29 | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.9 | - |
maltotriose | mutant Y172A, pH 6.0, 30°C | Corynebacterium glutamicum | |
19.6 | - |
maltotriose | wild-type, pH 6.0, 30°C | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
x * 81000, SDS-PAGE | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.2 | - |
mutant Y172A, pH 6.0, 30°C | Corynebacterium glutamicum |
21.8 | - |
wild-type, pH 6.0, 30°C | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltotriose | - |
Corynebacterium glutamicum | maltooligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 81000, SDS-PAGE | Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, almost complete loss of activity | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
maltotriose | mutant Y172A, pH 6.0, 30°C | Corynebacterium glutamicum | |
0.157 | - |
maltotriose | wild-type, pH 6.0, 30°C | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Corynebacterium glutamicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | stable at 30°C | Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
maltotriose | mutant Y172A, pH 6.0, 30°C | Corynebacterium glutamicum | |
0.0078 | - |
maltotriose | wild-type, pH 6.0, 30°C | Corynebacterium glutamicum |