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Literature summary for 2.4.1.25 extracted from

  • Srisimarat, W.; Kaulpiboon, J.; Krusong, K.; Zimmermann, W.; Pongsawasdi, P.
    Altered large-ring cyclodextrin product profile due to a mutation at Tyr-172 in the amylomaltase of Corynebacterium glutamicum (2012), Appl. Environ. Microbiol., 78, 7223-7228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
Y172A mutant exhibits lower disproportionation, cyclization, and hydrolysis activities than the wild-type. The kcat/Km of the disproportionation reaction for the Y172A enzyme is 2.8fold lower than that of wild-type. The Y172A enzyme shows a product pattern different from that of wild-type at a long incubation time. The principal large-ring cyclodextrin products of the Y172A mutant are a cycloamylose mixture with a degree of polymerization of 28 or 29 Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.9
-
maltotriose mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
19.6
-
maltotriose wild-type, pH 6.0, 30°C Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
x * 81000, SDS-PAGE Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2
-
mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
21.8
-
wild-type, pH 6.0, 30°C Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltotriose
-
Corynebacterium glutamicum maltooligosaccharides
-
?

Subunits

Subunits Comment Organism
? x * 81000, SDS-PAGE Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, almost complete loss of activity Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.036
-
maltotriose mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
0.157
-
maltotriose wild-type, pH 6.0, 30°C Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Corynebacterium glutamicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 stable at 30°C Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0028
-
maltotriose mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
0.0078
-
maltotriose wild-type, pH 6.0, 30°C Corynebacterium glutamicum