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Literature summary for 2.4.1.25 extracted from

  • Mun, S.; Kim, Y.L.; Kang, C.G.; Park, K.H.; Shim, J.Y.; Kim, Y.R.
    Development of reduced-fat mayonnaise using 4alphaGTase-modified rice starch and xanthan gum (2009), Int. J. Biol. Macromol., 44, 400-407.
    View publication on PubMed
Search for more literature for 2.4.1.25

Application

Application Comment Organism
food industry the disproportionating enzyme 4alphaGTase, is used to modify the structural properties of rice starch to produce a suitable fat substitute in reduced-fat mayonnaise. The mayonnaise fat is partially substituted with the 4alphaGTase-treated starch paste at levels up to 50% in combination with xanthan gum. All mayonnaises exhibit shear thinning behavior and yield stress. Viscoelastic properties of mayonnaise are altered, and the mayonnaises exhibited weak gel-like properties. The magnitude of elastic and loss moduli is also affected by 4alphaGTase-treated starch concentration and presence of xanthan gum, microstructure, method, overview Thermus aquaticus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by nickel affinity chromatography Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information modification of rice starch by the enzyme Thermus aquaticus ?
-
 ?

Synonyms

Synonyms Comment Organism
4alphaGTase
-
 Thermus aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 recombinant enzyme Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 the recombinant enzyme maintains more than 80% of its activity at a temperature range of 50-80°C and a pH range of 5.0-9.0 Thermus aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 recombinant enzyme Thermus aquaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 the recombinant enzyme maintains more than 80% of its activity at a temperature range of 50-80°C and a pH range of 5.0-9.0 Thermus aquaticus