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Literature summary for 2.4.1.25 extracted from

  • Godany, A.; Vidova, B.; Janecek, S.
    The unique glycoside hydrolase family 77 amylomaltase from Borrelia burgdorferi with only catalytic triad conserved (2008), FEMS Microbiol. Lett., 284, 84-91.
    View publication on PubMed
Search for more literature for 2.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
gene malQ, DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons, expression of the His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Borreliella burgdorferi

Organism

Organism UniProt Comment Textmining
Borreliella burgdorferi A6YM39 Lyme disease spirochaete, strain BB0166, gene malQ
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Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged MalQ from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Borreliella burgdorferi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MalQ is able to hydrolyse malto-oligosaccharides as well as to form their longer transglycosylation products and shows activity towards glucose, G1, and a series of malto-oligosaccharides, G2-G7, i.e. maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose Borreliella burgdorferi ?
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 ?

Synonyms

Synonyms Comment Organism
amylomaltase
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 Borreliella burgdorferi
GH77 amylomaltase
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 Borreliella burgdorferi
MalQ
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 Borreliella burgdorferi
additional information the enzyme belongs to the glycoside hydrolase family 77, together with GH13 and GH70, it forms the clan GH-H, known as the alpha-amylase family Borreliella burgdorferi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 recombinant enzyme Borreliella burgdorferi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 recombinant enzyme Borreliella burgdorferi