Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful in the enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain MC1061 | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-cyclodextrin | a mixed-type inhibitor | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.591 | - |
maltosyl-beta-cyclodextrin | pH 5.5, 75°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
gene treX | - |
Saccharolobus solfataricus P2 | - |
gene treX | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain MC1061 by nickel affinity chromatography | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + beta-cyclodextrin | condensation reaction mechanism, overview | Saccharolobus solfataricus | 6-O-alpha-maltosyl-beta-cyclodextrin | mass spectrometric product identification | ? | |
maltose + beta-cyclodextrin | condensation reaction mechanism, overview | Saccharolobus solfataricus P2 | 6-O-alpha-maltosyl-beta-cyclodextrin | mass spectrometric product identification | ? | |
maltose + maltosyl-beta-cyclodextrin | - |
Saccharolobus solfataricus | ? | - |
? | |
maltose + maltosyl-beta-cyclodextrin | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
additional information | synthesis of di-O-alpha-maltosyl-beta-cyclodextrin from 6-O-alpha-maltosyl-beta-cyclodextrin via a transglycosylation reaction, TreX transfers the maltosyl residue of a G2-beta-cyclodextrin to another molecule of G2-beta-cyclodextrin by forming an alpha-1,6-glucosidic linkage. TreX shows specificity for a branched glucosyl chain bigger than DP2 and no activity toward glucosyl-beta-cyclodextrin, transglycosylation reaction mechanism, overview | Saccharolobus solfataricus | ? | - |
? | |
additional information | synthesis of di-O-alpha-maltosyl-beta-cyclodextrin from 6-O-alpha-maltosyl-beta-cyclodextrin via a transglycosylation reaction, TreX transfers the maltosyl residue of a G2-beta-cyclodextrin to another molecule of G2-beta-cyclodextrin by forming an alpha-1,6-glucosidic linkage. TreX shows specificity for a branched glucosyl chain bigger than DP2 and no activity toward glucosyl-beta-cyclodextrin, transglycosylation reaction mechanism, overview | Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
debranching enzyme | - |
Saccharolobus solfataricus |
TreX | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 75 | assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0113 | - |
maltosyl-beta-cyclodextrin | pH 5.5, 75°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0556 | - |
beta-cyclodextrin | pH 5.5, 75°C, recombinant His-tagged enzyme | Saccharolobus solfataricus |