Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.25 extracted from

  • Bhuiyan, S.H.; Kitaoka, M.; Hayashi, K.
    A cycloamylose-forming hyperthermostable 4-alpha-glucanotransferase of Aquifex aeolicus expressed in Escherichia coli (2003), J. Mol. Catal. B, 22, 45-53.
No PubMed abstract available
Search for more literature for 2.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
 maltopentaose pH 6.6, 70°C Aquifex aeolicus
4.5
-
 maltotetraose pH 6.6, 70°C Aquifex aeolicus
4.5
-
 maltohexaose pH 6.6, 70°C Aquifex aeolicus
5.7
-
 maltoheptaose pH 6.6, 70°C Aquifex aeolicus
6.1
-
 maltotriose pH 6.6, 70°C Aquifex aeolicus
71
-
 maltose pH 6.6, 70°C Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aquifex aeolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44.2
-
-
 Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose the enzyme produces a cycloamylose with a minimum degree of polymerization of 16 Aquifex aeolicus cycloamylose
-
 ?
maltoheptaose + maltoheptaose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
maltohexaose + maltohexaose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
maltopentaose + maltopentaose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
maltose + maltose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
maltotetraose + maltotetraose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
maltotriose + maltotriose
-
 Aquifex aeolicus maltooligosaccharides
-
 ?
additional information the smallest acceptor is glucose, the smallest donor is maltose Aquifex aeolicus ?
-
 ?

Synonyms

Synonyms Comment Organism
4-alpha-glucanotransferase
-
 Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Aquifex aeolicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100 60°C: about 50% of maximal activity, 100°C: about 30% of maximal activity Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 30 min, less than 10% loss of activity Aquifex aeolicus
90
-
 30 min, about 30% loss of activity Aquifex aeolicus
100
-
 30 min, about 90% loss of activity Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 maltose pH 6.6, 70°C Aquifex aeolicus
16
-
 maltoheptaose pH 6.6, 70°C Aquifex aeolicus
17
-
 maltotetraose pH 6.6, 70°C Aquifex aeolicus
18
-
 maltotriose pH 6.6, 70°C Aquifex aeolicus
22
-
 maltopentaose pH 6.6, 70°C Aquifex aeolicus
22
-
 maltohexaose pH 6.6, 70°C Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Aquifex aeolicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 stable Aquifex aeolicus