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Literature summary for 2.4.1.25 extracted from

  • Imamura, H.; Fushinobu, S.; Yamamoto, M.; Kumasaka, T.; Jeon, B.S.; Wakagi, T.; Matsuzawa, H.
    Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor (2003), J. Biol. Chem., 278, 19378-19386.
    View publication on PubMed
Search for more literature for 2.4.1.25

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized in 2 forms, I and II, form I crystals belongs to hexagonal space group P6(4)22, form II crystals to orthorhombic space group P2(1)2(1)2 Thermococcus litoralis
hanging drop vapor diffusion method at 25°C, crystal structure of the enzyme with and without an inhibitor, acarbose. The acarbose-bound structure clarifies that Glu123 and Asp214 are the catalytic nucleophile and acid/base catalyst, respectively Thermococcus litoralis

Protein Variants

Protein Variants Comment Organism
D214N the specific activity of the D214N mutant is decreased about 10000fold as compared with that of the wild-type enzyme Thermococcus litoralis

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
 Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 2 * 78000, calculated from sequence Thermococcus litoralis
168000
-
 gel filtration Thermococcus litoralis

Organism

Organism UniProt Comment Textmining
Dictyoglomus thermophilum
-
-
-
Pyrococcus furiosus
-
-
-
Solanum tuberosum
-
-
-
Thermococcus kodakarensis
-
-
-
Thermococcus litoralis
-
-
-
Thermococcus litoralis O32462
-
-
Thermococcus litoralis DSM 5473 O32462
-
-
Thermus aquaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose + maltopentaose
-
 Thermus aquaticus cyclic alpha-1,4-glucan cycloamylose ?
amylose + maltopentaose
-
 Solanum tuberosum cyclic alpha-1,4-glucan cycloamylose ?
amylose + maltopentaose
-
 Thermococcus litoralis cyclic alpha-1,4-glucan cycloamylose ?
amylose + maltopentaose
-
 Thermococcus kodakarensis cyclic alpha-1,4-glucan
-
 ?
maltose + maltose key role in maltose metabolism Thermococcus litoralis maltotriose + glucose
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 78000, calculated from sequence Thermococcus litoralis

Synonyms

Synonyms Comment Organism
TLGT
-
 Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Thermococcus litoralis