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Literature summary for 2.4.1.25 extracted from

  • Van der Maarel, M.J.E.C.; Euverink, G.J.W.; Binnema, D.J.; Bos, H.T.P.; Bergsma, J.
    Amylomaltase from the hyperthermophilic bacterium Thermus thermophilus: Enzyme characteristics and application in the starch industry (2000), Meded. -Fac. Landbouwkd. Toegepaste Biol. Wet., 65, 231-234.
No PubMed abstract available

Application

Application Comment Organism
nutrition potential applications in the starch industry Thermus thermophilus
synthesis potential applications in the starch industry Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
malQ gene clone in pET expression vector system Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
maltotriose
-
Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
-
Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
potato
-
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
Thermus thermophilus maltooligosaccharides
-
?
1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
Solanum tuberosum maltooligosaccharides
-
?
1,4-alpha-D-glucan + glucose
-
Thermus thermophilus maltooligosaccharides
-
?
1,4-alpha-D-glucan + glucose
-
Solanum tuberosum maltooligosaccharides
-
?
maltotriose + maltotriose
-
Thermus thermophilus maltopentaose + maltotetraose
-
?
additional information glucose can only be used as acceptor of maltosyl units Thermus thermophilus ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Thermus thermophilus
monomer
-
Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
68
-
-
Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Thermus thermophilus