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Literature summary for 2.4.1.25 extracted from

  • Nakayama, A.; Yamamoto, K.; Tabata, S.
    High expression of glycogen-debranching enzyme in Escherichia coli and its competent purification method (2000), Protein Expr. Purif., 19, 298-303.
    View publication on PubMed
Search for more literature for 2.4.1.25

Application

Application Comment Organism
medicine understanding of the molecular basis of Cori's disease Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pTrc99A plasmid vector and expressed in Escherichia coli JM105 Saccharomyces cerevisiae
human glycogen debrancher gene assigned to chromosome 1p21, cloned and expressed in insect cells Homo sapiens
molecular cloning, sequencing and analysis of cDNA Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 phi-dextrin recombinant enzyme Saccharomyces cerevisiae
3
-
 phi-dextrin native enzyme Saccharomyces cerevisiae
10.5
-
 6-O-alpha-D-glucosyl-cyclomaltoheptaose native enzyme Saccharomyces cerevisiae
11.2
-
 6-O-alpha-D-glucosyl-cyclomaltoheptaose recombinant enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
165000
-
 purified recombinant enzyme, SDS-PAGE Saccharomyces cerevisiae
174900
-
 estimated from amino acid sequence, deduced from nucleotide sequence Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Gallus gallus
-
 chicken
-
Homo sapiens
-
 human
-
Oryctolagus cuniculus
-
 rabbit
-
Saccharomyces cerevisiae
-
 yeast
-
Squalus acanthias
-
 dogfish
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Oryctolagus cuniculus
native and recombinant enzyme Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
muscle skeletal muscle Gallus gallus
-
muscle skeletal muscle Homo sapiens
-
muscle skeletal muscle Oryctolagus cuniculus
-
muscle skeletal muscle Squalus acanthias
-
muscle pectoralis muscle Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.91
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, purified recombinant enzyme, 20 mM sodium phosphate buffer containing 15% glycerol, pH 7.0, no decrease in acivity observed for 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-alpha-D-glucosyl-cyclomaltoheptaose + H2O
-
 Saccharomyces cerevisiae D-glucose + cyclodextrins
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information 2 different enzyme activities, oligo-1,4-1,4-glucan-4-glycosyltransferase EC 2.4.1.25 and amylo-1,6-glucosidase EC 3.2.1.33 reside on the same polypeptide chain Saccharomyces cerevisiae ?
-
 ?
additional information 2 different enzyme activities, oligo-1,4-1,4-glucan-4-glycosyltransferase EC 2.4.1.25 and amylo-1,6-glucosidase EC 3.2.1.33 reside on the same polypeptide chain Oryctolagus cuniculus ?
-
 ?
phi-dextrin
-
 Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
GDE
-
 Saccharomyces cerevisiae
glycogen debranching enzyme
-
 Saccharomyces cerevisiae
oligo-1,4-1,4-glucantransferase
-
 Oryctolagus cuniculus
oligo-1,4-1,4-glucantransferase
-
 Saccharomyces cerevisiae