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Literature summary for 2.4.1.25 extracted from

  • Terada, Y.; Fujii, K.; Takaha, T.; Okada, S.
    Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization: production of cycloamylose (1999), Appl. Environ. Microbiol., 65, 910-915.
    View publication on PubMed
Search for more literature for 2.4.1.25

Application

Application Comment Organism
nutrition cycloamylose will be used in the food, pharmaceutical and chemical industries Thermus aquaticus
synthesis cycloamylose will be used in the food, pharmaceutical and chemical industries Thermus aquaticus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Chlamydia psittaci
-
 Clostridium butyricum
-
 Escherichia coli
-
 Solanum tuberosum
-
 Streptococcus pneumoniae
-
 Thermus aquaticus
cloned and sequenced and overexpressed in Escherichia coli Thermus aquaticus
putative gene identified in the genome Borreliella burgdorferi
putative gene identified in the genome Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57220
-
 amino acid sequence Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-
Borreliella burgdorferi
-
-
-
Chlamydia psittaci
-
-
-
Clostridium butyricum
-
 NCIMB 7423
-
Clostridium butyricum NCIMB 7423
-
 NCIMB 7423
-
Escherichia coli
-
-
-
Haemophilus influenzae
-
-
-
Hordeum vulgare
-
 barley
-
Mycobacterium tuberculosis
-
-
-
Solanum tuberosum
-
 potato
-
Streptococcus pneumoniae
-
-
-
Synechocystis sp.
-
-
-
Thermococcus litoralis
-
-
-
Thermus aquaticus
-
 ATCC 33923
-
Thermus aquaticus B7A9X4 ATCC 33923, nucleotide sequence accession number, deposited in DDBJ/EMBL/GenBank
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hordeum vulgare
-
 Thermococcus litoralis
native and recombinant enzyme Thermus aquaticus

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.9
-
-
 Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
 Thermus aquaticus maltooligosaccharides
-
 ?
1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
 Escherichia coli maltooligosaccharides
-
 ?
1,4-alpha-D-glucan + 1,4-alpha-D-glucan
-
 Solanum tuberosum maltooligosaccharides
-
 ?
1,4-alpha-D-glucan + glucose
-
 Thermus aquaticus maltooligosaccharides
-
 ?
1,4-alpha-D-glucan + glucose
-
 Escherichia coli maltooligosaccharides
-
 ?
1,4-alpha-D-glucan + glucose
-
 Solanum tuberosum maltooligosaccharides
-
 ?
amylose + maltopentaose
-
 Thermus aquaticus cyclic alpha-1,4-glucan cycloamylose ?
amylose + maltopentaose
-
 Solanum tuberosum cyclic alpha-1,4-glucan cycloamylose ?
amylose + maltopentaose
-
 Thermococcus litoralis cyclic alpha-1,4-glucan cycloamylose ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 stable up to, retains 50% activity after incubation at 80°C for 24 h, loses all activity after incubation at 100°C for 10 min Thermus aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Thermus aquaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10
-
 Thermus aquaticus