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Literature summary for 2.4.1.25 extracted from

  • Liebl, W.; Feil, R.; Gabelsberger, J.; Kellermann, J.; Schleifer, K.H.
    Purification and characterization of a novel thermostable 4-alpha-glucanotransferase of Thermotoga maritima cloned in Escherichia coli (1992), Eur. J. Biochem., 207, 81-88.
    View publication on PubMed
Search for more literature for 2.4.1.25

Activating Compound

Activating Compound Comment Organism Structure
maltoheptaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 124% Thermotoga maritima
maltohexaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 144% Thermotoga maritima
maltopentaose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% Thermotoga maritima
maltose stimulating effect of maltooligosaccharides on the conversion of amylose, relative activity 259% Thermotoga maritima
maltotetraose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 246% Thermotoga maritima
maltotriose stimulating effect of maltooligosaccharides on the conversion of amylose, 15 mM, relative activity 210% Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
chromosomal gene cloned and expressed in Escherichia coli and Corynebacterium glutamicum Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Thermotoga maritima 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 SDS-PAGE Thermotoga maritima
56000
-
 size exclusion chromatography Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltopentaose + maltopentaose Bacillus sp. (in: firmicutes) metabolism of starch in the bacterium maltooligosaccharides
-
 ?
maltopentaose + maltopentaose Thermotoga maritima metabolism of starch in the bacterium maltooligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: firmicutes)
-
-
-
Thermotoga maritima
-
 MSB8
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
 MSB8
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + maltopentaose
-
 Thermotoga maritima maltooligosaccharides
-
 ?
amylopectin + maltopentaose
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 maltooligosaccharides
-
 ?
amylose + maltopentaose
-
 Thermotoga maritima maltooligosaccharides
-
 ?
maltoheptaose + maltopentaose
-
 Bacillus sp. (in: firmicutes) maltooligosaccharides
-
 ?
maltoheptaose + maltopentaose
-
 Thermotoga maritima maltooligosaccharides
-
 ?
maltoheptaose + maltopentaose
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 maltooligosaccharides
-
 ?
maltohexaose + maltopentaose
-
 Bacillus sp. (in: firmicutes) maltooligosaccharides
-
 ?
maltohexaose + maltopentaose
-
 Thermotoga maritima maltooligosaccharides
-
 ?
maltohexaose + maltopentaose
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 maltooligosaccharides
-
 ?
maltopentaose + maltopentaose
-
 Bacillus sp. (in: firmicutes) new oligosaccharides
-
 ?
maltopentaose + maltopentaose
-
 Thermotoga maritima new oligosaccharides
-
 ?
maltopentaose + maltopentaose metabolism of starch in the bacterium Bacillus sp. (in: firmicutes) maltooligosaccharides
-
 ?
maltopentaose + maltopentaose metabolism of starch in the bacterium Thermotoga maritima maltooligosaccharides
-
 ?
maltotetraose + maltopentaose
-
 Thermotoga maritima maltooligosaccharides
-
 ?
maltotetraose + maltopentaose
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 maltooligosaccharides
-
 ?
additional information maltose and maltotriose are not disproportionated, glucose does not function as an acceptor sugar in transfer reactions, glucose also never appears as a reaction product Thermotoga maritima ?
-
 ?
additional information does not use maltose, maltotriose or maltotetraose as acceptor substrates in maltodextrinyltransfer reactions Bacillus sp. (in: firmicutes) ?
-
 ?
additional information alpha-D-glucose, beta-D-fructose, D-ribose, D-arabinose, D-xylose, isomaltose, D-trehalose, D-cellobiose, lactose, sucrose, raffinose and N-acetyl-D-glucosamine cannot participate as acceptor sugars in glucanosyl transfer from amylose Thermotoga maritima ?
-
 ?
starch + maltopentaose
-
 Bacillus sp. (in: firmicutes) alpha-1,4-D-glucans
-
 ?
starch + maltopentaose starch can serve as acceptor molecule in glycosyl transfer reactions Thermotoga maritima alpha-1,4-D-glucans
-
 ?
starch + maltopentaose starch can serve as acceptor molecule in glycosyl transfer reactions Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 alpha-1,4-D-glucans
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
GTase
-
 Thermotoga maritima
maltodextrin glycosyltransferase
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 80 stable at 37°C, retains more than 90% of its maximum activity between 55-80°C, half-life of activity of about 3 h at 80°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
3.5 10
-
 Thermotoga maritima