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Literature summary for 2.4.1.25 extracted from

  • Lin, T.P.; Preiss, J.
    Characterization of D-enzyme (4-alpha-glucanotransferase) in Arabidopsis leaf (1988), Plant Physiol., 86, 260-265.
    View publication on PubMed
Search for more literature for 2.4.1.25

Inhibitors

Inhibitors Comment Organism Structure
aplanin Bay e 4609, pseudooligosaccharide resembling amylose with a hydroxymethylconduritol unit and a 4-amino-4-deoxy-D-chinorose residue linked to a varying number of alpha-D-glucose units from 7-30 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 Columbia wild-type
-
Daucus carota
-
 carrot
-
Solanum lycopersicum
-
 tomato
-
Spinacia oleracea
-
 spinach
-
Vicia faba
-
 broad bean, L.major
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
leaf
-
 Spinacia oleracea
-
root
-
 Daucus carota
-
tuber
-
 Arabidopsis thaliana
-
tuber
-
 Solanum lycopersicum
-
tuber
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13
-
 D2 Arabidopsis thaliana
4.87
-
 D1 Arabidopsis thaliana

Storage Stability

Storage Stability Organism
4°C, stable in 20 mM bis-Tris-propane buffer, pH 6.5, containing 10% glycerol, no loss of activity over 6 months Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin + D-glucose
-
 Arabidopsis thaliana maltooligosaccharides
-
 ?
maltoheptaose + maltopentaose
-
 Arabidopsis thaliana maltooligosaccharides
-
 ?
maltotetraose + maltopentaose
-
 Arabidopsis thaliana maltooligosaccharides
-
 ?
maltotriose + maltotriose
-
 Arabidopsis thaliana maltopentaose + D-glucose
-
 ?
additional information 2 major forms, D1 and D2 Arabidopsis thaliana ?
-
 ?
additional information glucose is the major product of the D-enzyme reaction Vicia faba ?
-
 ?
additional information glucose is the major product of the D-enzyme reaction Spinacia oleracea ?
-
 ?
additional information glucose is the major product of the D-enzyme reaction Arabidopsis thaliana ?
-
 ?
additional information glucose is the major product of the D-enzyme reaction Daucus carota ?
-
 ?
additional information glucose is the major product of the D-enzyme reaction Solanum lycopersicum ?
-
 ?
additional information amylopectin is no substrate, maltose is not a product Arabidopsis thaliana ?
-
 ?
starch + glucose
-
 Spinacia oleracea maltotriose + ?
-
 ?
starch + glucose
-
 Arabidopsis thaliana maltotriose + ?
-
 ?
starch + glucose
-
 Daucus carota maltotriose + ?
-
 ?
starch + glucose
-
 Solanum lycopersicum maltotriose + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 loses activity when incubated for 10 min Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Daucus carota
5.5
-
-
 Solanum lycopersicum
6.5
-
-
 Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 no activity detected Arabidopsis thaliana