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Literature summary for 2.4.1.25 extracted from
- The isolation and mode of action of a bacterial glucanosyltransferase (1968), J. Biol. Chem., 243, 4732-4738.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | 80000 | sedimentation equilibrium | Bacillus sp. (in: Bacteria) |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltopentaose + maltopentaose | Bacillus sp. (in: Bacteria) | metabolism of starch in the bacterium | maltooligosaccharides | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus sp. (in: Bacteria) | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, relatively stable at low temperatures, may be stored for many months in a cold room with relatively little loss of activity | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltoheptaose + maltopentaose | - |
Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
| maltohexaose + maltopentaose | - |
Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
| maltopentaose + maltopentaose | - |
Bacillus subtilis | new oligosaccharides | - |
? | |
| maltopentaose + maltopentaose | - |
Bacillus amyloliquefaciens | new oligosaccharides | - |
? | |
| maltopentaose + maltopentaose | capable of transferring segments of 2 to 5 glucose residues linked by alpha-D-1-4 linkages from maltopentaose, higher molecular weight maltohomologues and starch to maltopentaose and other maltooligosaccharides | Bacillus sp. (in: Bacteria) | new oligosaccharides | - |
? | |
| maltopentaose + maltopentaose | metabolism of starch in the bacterium | Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
| additional information | glucose, maltose, maltotriose and maltotetraose are not acceptor molecules | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| starch + maltopentaose | - |
Bacillus sp. (in: Bacteria) | alpha-1,4-D-glucans | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
quite stable at low temperatures | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | loses 40% of the activity at pH 3.0, 80% at pH 8.0 | Bacillus sp. (in: Bacteria) |
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Release 2023.1 (January 2023)
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