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Literature summary for 2.4.1.25 extracted from

  • Xavier, K.; Peist, R.; Kossmann, M.; Boos, W.; Santos, H.
    Maltose metabolism in the hyperthermophilic archaeon Thermococcus litoralis Purification and characterization of key enzymes (1999), J. Bacteriol., 181, 3358-3367 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus litoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 maltoheptaose pH 7.0, 95°C Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
134000
-
 gel filtration Thermococcus litoralis

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis O32462 DSM 5473
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.7
-
 substrate: maltotriose, pH 7.0, 95°C Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose
-
 Thermococcus litoralis maltodextrin + D-glucose
-
 ?
maltose + D-glucose
-
 Thermococcus litoralis maltodextrin + D-glucose
-
 ?
maltotriose
-
 Thermococcus litoralis maltodextrin + D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 79000, SDS-PAGE Thermococcus litoralis

Synonyms

Synonyms Comment Organism
4-alpha-glucanotransferase
-
 Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Thermococcus litoralis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 105 70°C: about 60% of maximal activity, 105°C: about 50% of maximal activity Thermococcus litoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Thermococcus litoralis

pI Value

Organism Comment pI Value Maximum pI Value
Thermococcus litoralis isoelectric focusing
-
 3.5

Expression

Organism Comment Expression
Thermococcus litoralis the enzyme is constitutive additional information

General Information

General Information Comment Organism
metabolism key enzymes in the pathway for maltose catabolism. Glucose derived from the action of 4-alpha-glucanotransferase is subsequently metabolized via an Embden-Meyerhof pathway Thermococcus litoralis