Literature summary for 2.4.1.249 extracted from

Noda, N.; Yoshioka, S.; Kishimoto, S.; Nakayama, M.; Douzono, M.; Tanaka, Y.; Aida, R.
Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism (2017), Sci. Adv., 3, e1602785 .

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Application

Application	Comment	Organism
additional information	creation of blue flowers in ornamental plants. Two transgenes, namely, CamF3'5'H and CtA3'5'GT, are enough to generate blue chrysanthemum: The 3',5'-diglucosylated delphinidin exhibits a blue color by intermolecular association with flavone glucosides under the weakly acidic pH conditions of general flower petals	Clitoria ternatea

Cloned(Commentary)

Cloned (Comment)	Organism
Clitoria ternatea gene CtA3'5'GT encoding UDP-glucose:anthocyanin 3',5'-glucosyltransferase is introduced into Chrysanthemum by using binary vector pB249. Although transgenic plants expressing CtA3'5'GT are obtained, their flowers do not show a color change to bluish hue. Two transgenes, namely, CamF3'5'H and CtA3'5'GT, are enough to generate blue chrysanthemums: The 3',5'-diglucosylated delphinidin exhibits a blue color by intermolecular association with flavone glucosides under the weakly acidic pH conditions of general flower petals	Clitoria ternatea

Cloned (Comment)

Organism

Clitoria ternatea gene CtA3'5'GT encoding UDP-glucose:anthocyanin 3',5'-glucosyltransferase is introduced into Chrysanthemum by using binary vector pB249. Although transgenic plants expressing CtA3'5'GT are obtained, their flowers do not show a color change to bluish hue. Two transgenes, namely, CamF3'5'H and CtA3'5'GT, are enough to generate blue chrysanthemums: The 3',5'-diglucosylated delphinidin exhibits a blue color by intermolecular association with flavone glucosides under the weakly acidic pH conditions of general flower petals

Clitoria ternatea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside	Clitoria ternatea	the enzyme is involved in the anthocyanin biosynthetic pathway	2 UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3',5'-di-O-beta-D-glucoside	-	?

Organism

Organism	UniProt	Comment	Textmining
Clitoria ternatea	A4F1Q6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside	-	Clitoria ternatea	2 UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3',5'-di-O-beta-D-glucoside	-	?
2 UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside	the enzyme is involved in the anthocyanin biosynthetic pathway	Clitoria ternatea	2 UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3',5'-di-O-beta-D-glucoside	-	?

Synonyms

Synonyms	Comment	Organism
CtAA3'5'GT	-	Clitoria ternatea
UDP-glucose:anthocyanin 3',5'-glucosyltransferase	-	Clitoria ternatea
uridine diphosphate-glucose:anthocyanin 3',5'-glucosyltransferase	-	Clitoria ternatea

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the anthocyanin biosynthetic pathway	Clitoria ternatea