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Literature summary for 2.4.1.248 extracted from
- Carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides (2019), J. Microbiol. Biotechnol., 29, 1938-1946 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified | Thermoanaerobacter thermocopriae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
SDS-PAGE, the C-terminal region of TtCITase (Met740-Phe1559) | Thermoanaerobacter thermocopriae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter thermocopriae | WP_028992696 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified | Thermoanaerobacter thermocopriae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isomaltopentaose | the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates | Thermoanaerobacter thermocopriae | ? | - |
? |  |
| maltodextrin | the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates | Thermoanaerobacter thermocopriae | ? | - |
? | |
| maltopentaose | the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates | Thermoanaerobacter thermocopriae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CITase | - |
Thermoanaerobacter thermocopriae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Thermoanaerobacter thermocopriae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 80% of maximal activity, 50°C: about 50% of maximal activity | Thermoanaerobacter thermocopriae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermoanaerobacter thermocopriae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | pH 4.5: about 60% of maximal activity, pH 6.0: about 50% of maximal activity | Thermoanaerobacter thermocopriae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
18 h, enzyme retains 100% activity | Thermoanaerobacter thermocopriae |
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