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Literature summary for 2.4.1.245 extracted from
- Molecular cloning and characterization of trehalose synthase from Thermotoga maritima DSM3109 Syntheses of trehalose disaccharide analogues and NDP-glucoses (2010), Enzyme Microb. Technol., 47, 249-256 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is a useful thermostable enzyme particularly for the production of the trehalose analogue containing a mannose that acts as the inhibitor of intestinal sucrase | Thermotoga maritima |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 35% loss of activity | Thermotoga maritima |  |
| dimethylformamide | 5 mM, 79% loss of activity | Thermotoga maritima | |
| DMSO | 5 mM, 89% loss of activity | Thermotoga maritima | |
| ethanol | 5 mM, 47% loss of activity | Thermotoga maritima | |
| methanol | 5 mM, 36% loss of activity | Thermotoga maritima | |
| Mn2+ | 5 mM, 82% loss of activity | Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
| 1.7 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
| 2.3 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
| 40 | - |
ADP | pH 6.0, 60°C | Thermotoga maritima | |
| 54 | - |
GDP | pH 6.0, 60°C | Thermotoga maritima | |
| 71 | - |
UDP | pH 6.0, 60°C | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 5 mM, 1.2fold activation | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
SDS-PAGE | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
| Thermotoga maritima DSM 3109 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima | alpha,alpha-trehalose + ADP | - |
r | |
| ADP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima DSM 3109 | alpha,alpha-trehalose + ADP | - |
r | |
| alpha,alpha-trehalose + ADP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | ADP-alpha-D-glucose + D-glucose | - |
r | |
| alpha,alpha-trehalose + ADP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima DSM 3109 | ADP-alpha-D-glucose + D-glucose | - |
r | |
| alpha,alpha-trehalose + CDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | CDP-alpha-D-glucose + D-glucose | - |
r | |
| alpha,alpha-trehalose + GDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | GDP-alpha-D-glucose + D-glucose | - |
? | |
| alpha,alpha-trehalose + GDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima DSM 3109 | GDP-alpha-D-glucose + D-glucose | - |
? | |
| alpha,alpha-trehalose + UDP | both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | UDP-alpha-D-glucose + D-glucose | - |
? | |
| CDP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima | alpha,alpha-trehalose + CDP | - |
r | |
| GDP-alpha-D-glucose + D-glucose | - |
Thermotoga maritima | alpha,alpha-trehalose + GDP | - |
r | |
| additional information | the enzyme does not utilize galactose as an acceptor | Thermotoga maritima | ? | - |
? | |
| additional information | the enzyme does not utilize galactose as an acceptor | Thermotoga maritima DSM 3109 | ? | - |
? | |
| UDP-alpha-D-glucose + D-fructose | the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction | Thermotoga maritima | ? | - |
r | |
| UDP-alpha-D-glucose + D-glucose | the purified soluble enzyme, as well as the glutathione-S-transferase-fusion enzyme, show the catalytic specificity for synthesizing trehalose from UDP-alpha-D-glucose as the donor and glucose as the acceptor. The enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. Both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order | Thermotoga maritima | alpha,alpha-trehalose + UDP | - |
r | |
| UDP-alpha-D-glucose + D-mannose | the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction | Thermotoga maritima | ? | - |
r | |
| UDP-alpha-D-glucose + D-mannose | the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction | Thermotoga maritima DSM 3109 | ? | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TmTreT | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 70 | 35°C: about 40% of maximal activity, 70°C: about 70% of maximal activity | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
2 h, activity of the the glutathione-S-transferase-fusion enzyme is fully maintained | Thermotoga maritima |
| 60 | - |
2 h, 10% decrease of the activity of the the glutathione-S-transferase-fusion enzyme, activity of the soluble enzyme is decreased by 35% | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.1 | - |
UDP | pH 6.0, 60°C | Thermotoga maritima | |
| 9.7 | - |
GDP | pH 6.0, 60°C | Thermotoga maritima | |
| 11.1 | - |
ADP | pH 6.0, 60°C | Thermotoga maritima | |
| 20 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
| 25 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
| 34 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | pH 4.5: about 45% of maximal activity, pH 8.0: about 40% of maximal activity | Thermotoga maritima |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | activity is relatively stable in the wide pH range of 5.0-10.0 | Thermotoga maritima |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
UDP | pH 6.0, 60°C | Thermotoga maritima | |
| 0.2 | - |
GDP | pH 6.0, 60°C | Thermotoga maritima | |
| 0.3 | - |
ADP | pH 6.0, 60°C | Thermotoga maritima | |
| 9 | - |
ADP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
| 15 | - |
UDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
| 20 | - |
GDP-alpha-D-glucose | pH 6.0, 60°C | Thermotoga maritima | |
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