Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rubrobacter xylanophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | 2 mM | Rubrobacter xylanophilus | |
Zn2+ | 2 mM | Rubrobacter xylanophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
ADP-glucose | recombinant enzyme, at 60°C | Rubrobacter xylanophilus | |
1.3 | - |
D-glucose | recombinant enzyme, at 60°C | Rubrobacter xylanophilus | |
6.8 | - |
ADP | recombinant enzyme, at 60°C | Rubrobacter xylanophilus | |
82 | - |
alpha,alpha-1,1-trehalose | recombinant enzyme, at 60°C | Rubrobacter xylanophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM or 20 mM Ca2+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
Fe3+ | 2 mM Fe3+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
Li+ | 2 mM or 20 mM Li+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
Mg2+ | Mg2+ is required for activity, 20 mM Mg+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
Mn2+ | 2 mM Mn2+ induces slightly enhanced activity | Rubrobacter xylanophilus | |
additional information | no enhanced activity in the presence of 20 mM Fe3+ or Mn2+ | Rubrobacter xylanophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46700 | - |
calculated from amino acid sequence | Rubrobacter xylanophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubrobacter xylanophilus | Q1ARU5 | strain PRD-1T (DSM 9941) | - |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, Q-Sepharose fast-flow column chromatography, and Superdex 200 gel filtration | Rubrobacter xylanophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose | 100% activity | Rubrobacter xylanophilus | ADP + alpha,alpha-1,1-trehalose | - |
? | |
GDP-glucose + D-glucose | 48.2% activity compared to ADP-glucose | Rubrobacter xylanophilus | alpha,alpha-1,1-trehalose + GDP | - |
? | |
UDP-glucose + D-glucose | 32.7% activity compared to ADP-glucose | Rubrobacter xylanophilus | alpha,alpha-1,1-trehalose + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trehalose glycosyltransferring synthase | - |
Rubrobacter xylanophilus |
TreT | - |
Rubrobacter xylanophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Rubrobacter xylanophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Rubrobacter xylanophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the half-lives for inactivation at 60°C and at 70°C are 309 h and 4.1 h, respectively | Rubrobacter xylanophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Rubrobacter xylanophilus |