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Literature summary for 2.4.1.245 extracted from

  • Nobre, A.; Alarico, S.; Fernandes, C.; Empadinhas, N.; da Costa, M.S.
    A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT (2008), J. Bacteriol., 190, 7939-7946.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rubrobacter xylanophilus

Inhibitors

Inhibitors Comment Organism Structure
Ni2+ 2 mM Rubrobacter xylanophilus
Zn2+ 2 mM Rubrobacter xylanophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
ADP-glucose recombinant enzyme, at 60°C Rubrobacter xylanophilus
1.3
-
D-glucose recombinant enzyme, at 60°C Rubrobacter xylanophilus
6.8
-
ADP recombinant enzyme, at 60°C Rubrobacter xylanophilus
82
-
alpha,alpha-1,1-trehalose recombinant enzyme, at 60°C Rubrobacter xylanophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM or 20 mM Ca2+ induces slightly enhanced activity Rubrobacter xylanophilus
Fe3+ 2 mM Fe3+ induces slightly enhanced activity Rubrobacter xylanophilus
Li+ 2 mM or 20 mM Li+ induces slightly enhanced activity Rubrobacter xylanophilus
Mg2+ Mg2+ is required for activity, 20 mM Mg+ induces slightly enhanced activity Rubrobacter xylanophilus
Mn2+ 2 mM Mn2+ induces slightly enhanced activity Rubrobacter xylanophilus
additional information no enhanced activity in the presence of 20 mM Fe3+ or Mn2+ Rubrobacter xylanophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46700
-
calculated from amino acid sequence Rubrobacter xylanophilus

Organism

Organism UniProt Comment Textmining
Rubrobacter xylanophilus Q1ARU5 strain PRD-1T (DSM 9941)
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, Q-Sepharose fast-flow column chromatography, and Superdex 200 gel filtration Rubrobacter xylanophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose 100% activity Rubrobacter xylanophilus ADP + alpha,alpha-1,1-trehalose
-
?
GDP-glucose + D-glucose 48.2% activity compared to ADP-glucose Rubrobacter xylanophilus alpha,alpha-1,1-trehalose + GDP
-
?
UDP-glucose + D-glucose 32.7% activity compared to ADP-glucose Rubrobacter xylanophilus alpha,alpha-1,1-trehalose + UDP
-
?

Synonyms

Synonyms Comment Organism
trehalose glycosyltransferring synthase
-
Rubrobacter xylanophilus
TreT
-
Rubrobacter xylanophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Rubrobacter xylanophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
Rubrobacter xylanophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the half-lives for inactivation at 60°C and at 70°C are 309 h and 4.1 h, respectively Rubrobacter xylanophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10
-
Rubrobacter xylanophilus