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Literature summary for 2.4.1.243 extracted from

  • Lasseur, B.; Schroeven, L.; Lammens, W.; Le Roy, K.; Spangenberg, G.; Manduzio, H.; Vergauwen, R.; Lothier, J.; Prudhomme, M.P.; Van den Ende, W.
    Transforming a fructan:fructan 6G-fructosyltransferase from perennial ryegrass into a sucrose:sucrose 1-fructosyltransferase (2009), Plant Physiol., 149, 327-339.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.243

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Lolium perenne

Protein Variants

Protein Variants Comment Organism
N340D/W343R the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants Lolium perenne
S415N the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected Lolium perenne

General Stability

General Stability Organism
several attempts to purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast’s expression medium Lolium perenne

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-kestose + 3 sucrose Lolium perenne
-
 3 neokestose + H2O
-
 ?
4 1-kestose Lolium perenne
-
 sucrose + 1G-kestotetraose + 6G-kestotetraose + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lolium perenne Q8S337
-
-
Lolium perenne Q8S337 perennial ryegrass
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast’s expression medium Lolium perenne

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + 1-kestose 6G-FFT activity Lolium perenne D-sucrose + 1G-kestotetraose + 6G-kestotetraose
-
 ?
1-kestose + 3 sucrose
-
 Lolium perenne 3 neokestose + H2O
-
 ?
1-kestose + sucrose 6G-FFT activity Lolium perenne D-sucrose + neokestose
-
 ?
4 1-kestose
-
 Lolium perenne sucrose + 1G-kestotetraose + 6G-kestotetraose + H2O
-
 ?

Synonyms

Synonyms Comment Organism
6G-FFT
-
 Lolium perenne
6G-FFT/1-FFT
-
 Lolium perenne
fructan:fructan 6G-fructosyltransferase
-
 Lolium perenne
fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase
-
 Lolium perenne