Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | 1 mM, 209% of initial activity | Allium cepa | |
Triton X-100 | 1 mM, 160% of initial activity | Allium cepa | |
Tween-80 | 1mM, 171% of initial activity | Allium cepa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, no residual activity | Allium cepa | |
Hg2+ | 1 mM, no residual activity | Allium cepa | |
p-chloromercuribenzoate | 0.1 mM, 2.5% residual activity | Allium cepa | |
Sodium dodecyl sulfate | 1 mM, 48% residual activity | Allium cepa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
88 | - |
1F(1-beta-D-fructofuranosyl)sucrose | pH 5.7, 30°C | Allium cepa | |
310 | - |
1F(1-beta-D-fructofuranosyl)2 sucrose | pH 5.7, 30°C | Allium cepa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 52000, x * 25000, SDS-PAGE | Allium cepa |
52000 | - |
x * 52000, x * 25000, SDS-PAGE | Allium cepa |
66000 | - |
gel filtration | Allium cepa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Allium cepa | plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allium cepa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Allium cepa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bulb | - |
Allium cepa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.54 | - |
pH 5.68, 30°C | Allium cepa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1F(1-beta-D-fructofuranosyl)2 sucrose + sucrose | i.e. nystose | Allium cepa | 1F,6G-di-beta-D-fructofuranosylsucrose + ? | - |
? | |
1F(1-beta-D-fructofuranosyl)3 sucrose + sucrose | i.e. fructosyl nystose | Allium cepa | 1F,6G-di-beta-D-fructofuranosylsucrose + ? | - |
? | |
1F(1-beta-D-fructofuranosyl)sucrose + sucrose | i.e. 1-kestose | Allium cepa | 1F,6G-di-beta-D-fructofuranosylsucrose + ? | - |
? | |
additional information | plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides | Allium cepa | ? | - |
? | |
neokestose + sucrose | - |
Allium cepa | 1F,6G-di-beta-D-fructofuranosylsucrose + ? | - |
? | |
sucrose + sucrose | - |
Allium cepa | 1F,6G-di-beta-D-fructofuranosylsucrose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, x * 25000, SDS-PAGE | Allium cepa |
More | N-terminal amino acid sequence of 52000 Da polypeptide and of 25000 Da polypeptide | Allium cepa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | stable for at least 15 min | Allium cepa |
50 | - |
inactivation | Allium cepa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
- |
Allium cepa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 6.3 | more than 80% of maximum activity within this range | Allium cepa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.3 | 6.3 | 30°C, stable for 30 min | Allium cepa |