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Literature summary for 2.4.1.241 extracted from

  • Fu, B.; Brown, C.; Maeler, L.
    Expression and purification of DGD2, a chloroplast outer membrane-associated glycosyltransferase for galactolipid synthesis (2020), Biochemistry, 59, 999-1009 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Lipid activity is stimulated by the presence of negatively charged lipids, which highlight the importance of the membrane environment in modulating the activity of the enzyme Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
DELTA401-473 removal of around 70 C-terminal residues is necessary to produce a homogeneous monomeric protein sample with high purity, which was highly active. The active form of the enzyme does not require the 73 C-terminal amino acid residues Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast outer membrane Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not require Mg2+ Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactose + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol Arabidopsis thaliana
-
UDP + 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8W1S1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the active form of the enzyme does not require the 73 C-terminal amino acid residues. They may be cleaved off in vivo after import into the chloroplast Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
removal of around 70 C-terminal residues is necessary to produce a homogeneous monomeric protein sample with high purity, which was highly active Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactose + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
Arabidopsis thaliana UDP + 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol
-
?

Synonyms

Synonyms Comment Organism
DGD2
-
Arabidopsis thaliana
digalactosyldiacylglycerol synthase 2
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana gene expression is significantly upregulated by phosphate starvation up