BRENDA - Enzyme Database
show all sequences of 2.4.1.241

Reduced bosynthesis of digalactosyldiacylglycerol, a major chloroplast membrane lipid, leads to oxylipin overproduction and phloem cap lignification in Arabidopsis

Lin, Y.T.; Chen, L.J.; Herrfurth, C.; Feussner, I.; Li, H.M.; Plant Cell 28, 219-232 (2016)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9S7D1
-
-
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
General Information
General Information
Commentary
Organism
physiological function
Dgd1 mutants have a greater than 90% reduction in digalactosyldiacylglycerol content, reduced photosynthesis, and altered chloroplast morphology. Mutant plants show an extremely short inflorescence stem. Phloem cap cells are lignified and jasmonic acid-responsive genes are highly upregulated under normal growth conditions. The coronative insensitive1 Dgd1 and allene oxide synthase Dgd1 double mutants no longer exhibit the short inflorescence stem and lignification phenotypes but still have the same lipid profile and reduced photosynthesis as Dgd1 single mutants. Dgd1 mutants display increased levels of jasmonic acid, jasmonic acid-isoleucine, 12-oxo-phytodienoic acid, and arabidopsides. Jasmonic acid biosynthesis in Dgd1 mutants is initially activated through the increased expression of genes encoding 13-lipoxygenases and phospholipase A-Ig3 (At1g51440), and is sustained by further increases in 13-lipoxygenase and allene oxide cyclase mRNA and protein levels
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Dgd1 mutants have a greater than 90% reduction in digalactosyldiacylglycerol content, reduced photosynthesis, and altered chloroplast morphology. Mutant plants show an extremely short inflorescence stem. Phloem cap cells are lignified and jasmonic acid-responsive genes are highly upregulated under normal growth conditions. The coronative insensitive1 Dgd1 and allene oxide synthase Dgd1 double mutants no longer exhibit the short inflorescence stem and lignification phenotypes but still have the same lipid profile and reduced photosynthesis as Dgd1 single mutants. Dgd1 mutants display increased levels of jasmonic acid, jasmonic acid-isoleucine, 12-oxo-phytodienoic acid, and arabidopsides. Jasmonic acid biosynthesis in Dgd1 mutants is initially activated through the increased expression of genes encoding 13-lipoxygenases and phospholipase A-Ig3 (At1g51440), and is sustained by further increases in 13-lipoxygenase and allene oxide cyclase mRNA and protein levels
Arabidopsis thaliana
Other publictions for EC 2.4.1.241
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735719
Maida
Digalactosyldiacylglycerol is ...
Synechococcus elongatus
Biochim. Biophys. Acta
1861
1309-1314
2016
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736994
Lin
Reduced bosynthesis of digalac ...
Arabidopsis thaliana
Plant Cell
28
219-232
2016
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706159
Ingle
Chloroplast biogenesis during ...
Xerophyta humilis
Plant Cell Environ.
31
1813-1824
2008
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689403
Torres-Franklin
Drought stress and rehydration ...
Vigna unguiculata
Physiol. Plant.
131
201-210
2007
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689486
Awai
Digalactosyldiacylglycerol is ...
Synechocystis sp. PCC 6803
Plant Cell Physiol.
48
1517-1523
2007
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1
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6
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659479
Benning
Three enzyme systems for galac ...
Arabidopsis sp.
J. Biol. Chem.
280
2397-2400
2005
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682371
Hofmann
Protein- and energy-mediated t ...
Physcomitrella patens
Plant J.
44
917-927
2005
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660126
Kelly
Disruption of the two digalact ...
Arabidopsis sp.
Plant Cell
15
2694-2706
2003
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659169
Kelly
DGD2, an Arabidopsis gene enco ...
Arabidopsis sp.
J. Biol. Chem.
277
1166-1173
2002
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659157
Froehlich
The digalactosyldiacylglycerol ...
Arabidopsis sp.
J. Biol. Chem.
276
31806-31812
2001
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660402
Härtel
DGD1-independent biosynthesis ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
97
10649-10654
2000
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