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Literature summary for 2.4.1.239 extracted from

  • Jourdan, P.S.; Mansell, R.L.
    Isolation and partial characterization of three glucosyl transferases involved in the biosynthesis of flavonol triglucosides in Pisum sativum L (1982), Arch. Biochem. Biophys., 213, 434-443.
    View publication on PubMed
Search for more literature for 2.4.1.239

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by divalent cations, 0.1-10 mM, EDTA, EGTA, no substrate inhibition Pisum sativum
UDP product inhibition, 1.75 mM, 50% inhibition Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 kaempferol 3-O-beta-D-glucoside pH 7.3, 30°C Pisum sativum
0.64
-
 UDP-glucose pH 7.3, 30°C Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by divalent cations, 0.1-10 mM Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pisum sativum one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 var. Alaska
-

Purification (Commentary)

Purification (Comment) Organism
331fold, separation of three distinct flavonol:glucosyltransferases Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling light-grown, one week old Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pisum sativum

Storage Stability

Storage Stability Organism
-15°C or room temperature, partially purified enzyme, 36 h, 80% loss of activity Pisum sativum
4°C, 2 weeks, stable Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides Pisum sativum ?
-
 ?
additional information no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol diglucoside, quercetin-3-rutinoside, quercetin-3-rhamnoside Pisum sativum ?
-
 ?
TDP-glucose + a flavonol 3-O-beta-D-glucoside 3fold lower rate than with UDP-glucose as glucosyl donor Pisum sativum TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
UDP-glucose + a flavonol 3-O-beta-D-glucoside highly specific enzyme, specific for flavonol-3-O-monoglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor Pisum sativum UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
UDP-glucose + kaempferol 3-O-beta-D-glucoside best substrate Pisum sativum UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
UDP-glucose + myricetin 3-O-beta-D-glucoside 50% of the activity with kaempferol 3-O-beta-D-glucoside Pisum sativum UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
UDP-glucose + pelargonidin 3-O-beta-D-glucoside 2% of the activity with kaempferol 3-O-beta-D-glucoside Pisum sativum UDP + pelargonidin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?
UDP-glucose + quercetin 3-O-beta-D-glucoside 62% of the activity with kaempferol 3-O-beta-D-glucoside Pisum sativum UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
UDP-glucose:glucosyltransferase
-
 Pisum sativum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 0.2 M glycine-NaOH buffer, lower activity in 0.2 M Tris-HCl buffer Pisum sativum

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Pisum sativum