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Literature summary for 2.4.1.236 extracted from
- Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast (2016), Appl. Microbiol. Biotechnol., 100, 687-696.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determiation and analysis, phylogenetic analysis, recombinant expression of C-terminally His6-Myc-tagged enzyme in Nicotiana tabacum BY-2 cells | Citrus maxima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | production of naringin and narirutin from naringenin-7-O-glucoside using the engineered Schizosaccharomyces expressing Arabidopsis thaliana rhamnose synthase 2, AtRHM2, and glycosyltransferases Cm1,2RhaT from Citrus maxima or Cs1,6RhaT from Citrus sinensis as a whole-cell-biocatalyst, overview | Citrus maxima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, recombinant C-terminally His6-Myc-tagged enzyme, SDS-PAGE | Citrus maxima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus maxima | A0A0P0YNP7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Cm1,2RhaT has glycosyl transfer activity toward UDP-xylose as well as UDP-rhamnose, it is a branch-forming rhamnosyltransferase, substrate specificity of the recombinant enzyme, overview. No activity with quercetin-3-O-glucoside | Citrus maxima | ? | - |
? |  |
| UDP-beta-L-rhamnose + a flavanone 7-O-beta-D-glucoside | - |
Citrus maxima | UDP + a flavanone 7-O-[alpha-L-rhamnosyl-(1->2)-beta-D-glucoside] | - |
? | |
| UDP-beta-L-rhamnose + naringenin-7-O-glucoside | - |
Citrus maxima | UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->2)-beta-D-glucoside] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, recombinant C-terminally His6-Myc-tagged enzyme, SDS-PAGE | Citrus maxima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cm1,2RhaT | - |
Citrus maxima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Citrus maxima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Citrus maxima |
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Release 2023.1 (January 2023)
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