Application | Comment | Organism |
---|---|---|
nutrition | the isolation of the rhamnosyltransferase gene may enable its use in genetic engineering directed to modifying grapefruit bitterness | Citrus maxima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | inhibits the activity of the partially purified enzyme | Citrus maxima | |
apigenin 7-O-neohesperidoside | 0.1 mM, 45% inhibition | Citrus maxima | |
bromo-naphthyl-beta-glucopyranoside | 1 mM, 80% inhibition | Citrus maxima | |
EGTA | weak, 30 mM, 20% inhibition | Citrus maxima | |
hesperetin | 1 mM: 66% inhibition, 0.1 mM: 47% inhibition | Citrus maxima | |
additional information | not inhibited by neohesperidin, hesperidin, EDTA, arbutin, nitrophenyl-beta-glucopyranoside, nitrophenyl-L-rhamnopyranoside | Citrus maxima | |
naringenin | 1 mM: 72% inhibition, 0.1 mM: 54% inhibition | Citrus maxima | |
naringin | weak, 0.1 mM, 12% inhibition | Citrus maxima | |
quercetin | 0.1 mM: 93% inhibition, 0.01 mM: 55% inhibition | Citrus maxima | |
UDP | product inhibition, 0.01 mM: 62% inhibition, 0.1 mM: 93% inhibition | Citrus maxima | |
UTP | 0.1 mM, 83% inhibition | Citrus maxima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
UDP-L-rhamnose | pH 7.5, 37°C, cosubstrate hesperetin 7-O-glucoside | Citrus maxima | |
0.0013 | - |
UDP-L-rhamnose | pH 7.5, 37°C, cosubstrate naringenin 7-O-glucoside | Citrus maxima | |
0.0024 | - |
naringenin 7-O-glucoside | pH 7.5, 37°C | Citrus maxima | |
0.0415 | - |
hesperetin 7-O-glucoside | pH 7.5, 37°C | Citrus maxima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Citrus maxima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, SDS-PAGE | Citrus maxima |
52000 | - |
gel filtration | Citrus maxima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Citrus maxima | enzyme catalyzes the production of bitter flavanone-glucosides | ? | - |
? | |
UDP-L-rhamnose + naringenin 7-O-glucoside | Citrus maxima | i.e. prunin, natural substrate | UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus maxima | - |
pummelo, grown in Kibbutz Naan | - |
Purification (Comment) | Organism |
---|---|
2735fold | Citrus maxima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-beta-L-rhamnose + a flavanone 7-O-beta-D-glucoside = UDP + a flavanone 7-O-[alpha-L-rhamnosyl-(1->2)-beta-D-glucoside] | acts on the 7-O-glucoside of naringenin and hesperetin, also the flavone 7-O-glucosides of luteolin and apigenin | Citrus maxima |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young, 0.5-2 cm long | Citrus maxima | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000624 | - |
pH 7.5, 37°C | Citrus maxima |
Storage Stability | Organism |
---|---|
-70°C, 20-50 mM Tris-HCl, pH 7.5, months, stable | Citrus maxima |
4°C, 24 h, over 80% loss of activity | Citrus maxima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme catalyzes the production of bitter flavanone-glucosides | Citrus maxima | ? | - |
? | |
additional information | not: flavonoid 5-O-glucosides or aglycones, naringenin, hesperetin, quercetin, UDP-glucose, UDP-galactose, UDP-glucoronic acid | Citrus maxima | ? | - |
? | |
UDP-L-rhamnose + apigenin 7-O-glucoside | 98% of the activity with hesperetin 7-O-glucoside as substrate, highly specific for UDP-rhamnose | Citrus maxima | UDP + apigenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | - |
? | |
UDP-L-rhamnose + hesperetin 7-O-glucoside | enzyme catalyzes the transfer of rhamnose from UDP-rhamnose to the C-2 hydroxyl group of glucose attached via C-7-O- of naringenin or hesperetin, lower activity than with naringenin 7-O-glucoside as substrate, highly specific for UDP-rhamnose | Citrus maxima | UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. neohesperidin | ? | |
UDP-L-rhamnose + luteolin 7-O-glucoside | 99% of the activity with hesperetin 7-O-glucoside as substrate, highly specific for UDP-rhamnose | Citrus maxima | UDP + luteolin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | - |
? | |
UDP-L-rhamnose + naringenin 7-O-glucoside | i.e. prunin, natural substrate | Citrus maxima | UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | - |
? | |
UDP-L-rhamnose + naringenin 7-O-glucoside | i.e. prunin, natural substrate, enzyme catalyzes the transfer of rhamnose from UDP-rhamnose to the C-2 hydroxyl group of glucose attached via C-7-O- of naringenin or hesperetin, best substrate, 141% of the activity with hesperetin 7-O-glucoside as substrate, highly specific for UDP-rhamnose | Citrus maxima | UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] | i.e. naringin | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Citrus maxima |
Synonyms | Comment | Organism |
---|---|---|
1-2 UDP-rhamnosyltransferase | - |
Citrus maxima |
rhamnosyltransferase, uridine diphosphorhamnose-flavanone 7-O-glucoside 2''-O- | - |
Citrus maxima |
UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase | - |
Citrus maxima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Citrus maxima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Citrus maxima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | below pH 6: 90% loss of activity, above pH 8.5: 50% loss of activity | Citrus maxima |