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Literature summary for 2.4.1.234 extracted from

  • Miller, K.D.; Guyon, V.; Evans, J.N.S.; Shuttleworth, W.A.; Taylor, L.P.
    Purification, cloning, and heterologous expression of a catalytically efficient flavonol 3-O-galactosyltransferase expressed in the male gametophyte of Petunia hybrida (1999), J. Biol. Chem., 274, 34011-34019.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
from pollen, full-length cDNA, expression in Escherichia coli, sequencing Petunia x hybrida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00096
-
quercetin pH 7.5, 43°C, forward reaction Petunia x hybrida
0.0011
-
kaempferol pH 7.5, 43°C, forward reaction Petunia x hybrida
0.00265
-
quercetin 3-O-galactoside pH 6, 43°C, reverse reaction Petunia x hybrida
0.00847
-
kaempferol 3-O-beta-D-galactoside pH 6, 43°C, reverse reaction Petunia x hybrida
0.066
-
UDP pH 6, 43°C, cosubstrate kaempferol 3-O-galactoside, reverse reaction Petunia x hybrida
0.102
-
UDP pH 6, 43°C, cosubstrate quercetin 3-O-galactoside, reverse reaction Petunia x hybrida
0.387
-
UDP-galactose pH 7.5, 43°C, cosubstrate quercetin, forward reaction Petunia x hybrida
0.426
-
UDP-galactose pH 7.5, 43°C, cosubstrate kaempferol, forward reaction Petunia x hybrida

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant F3GalTase Petunia x hybrida
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 45000, native and recombinant F3GalTase, SDS-PAGE, x * 48900, sequence calculation Petunia x hybrida
48900
-
1 * 45000, native and recombinant F3GalTase, SDS-PAGE, x * 48900, sequence calculation Petunia x hybrida
50000
-
gel filtration, native PAGE Petunia x hybrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + flavonol Petunia x hybrida F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen UDP + flavonol 3-O-beta-D-galactoside
-
?
UDP-galactose + flavonol Petunia x hybrida CMF F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen UDP + flavonol 3-O-beta-D-galactoside
-
?

Organism

Organism UniProt Comment Textmining
Petunia x hybrida Q9SBQ8 CMF
-
Petunia x hybrida CMF Q9SBQ8 CMF
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification residues 1-30 potentially comprise a cleavable signal sequence Petunia x hybrida

Purification (Commentary)

Purification (Comment) Organism
native F3GalTase Petunia x hybrida

Source Tissue

Source Tissue Comment Organism Textmining
gametophyte F3GalTase is expressed exclusively in the male gametophyte/pollen Petunia x hybrida
-
pollen from anthers of stage 2-9 flowers, F3GalTase is expressed exclusively in the male gametophyte/pollen, where it modifies a molecule required for functional pollen, expression pattern during development Petunia x hybrida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: apigenin, naringenin, dihydroquercetin, pelargonidin, delphinidin, cyanidin, NAD+, ferrulic acid, sinapic acid, naphthylphthamic acid, gibberillic acid, indole acetic acid, abscisic acid, jasmonic acid, not in the reverse direction: kaempferol 3-O-glucoside, quercetin 3-O-glucoside Petunia x hybrida ?
-
?
additional information not: apigenin, naringenin, dihydroquercetin, pelargonidin, delphinidin, cyanidin, NAD+, ferrulic acid, sinapic acid, naphthylphthamic acid, gibberillic acid, indole acetic acid, abscisic acid, jasmonic acid, not in the reverse direction: kaempferol 3-O-glucoside, quercetin 3-O-glucoside Petunia x hybrida CMF ?
-
?
UDP + kaempferol 3-O-beta-D-galactoside
-
Petunia x hybrida UDP-alpha-D-galactose + kaempferol
-
r
UDP-galactose + fisetin 23.3% of the activity with kaempferol as substrate Petunia x hybrida UDP + fisetin 3-O-beta-D-galactoside
-
r
UDP-galactose + flavonol F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen Petunia x hybrida UDP + flavonol 3-O-beta-D-galactoside
-
?
UDP-galactose + flavonol F3GalTase uses only UDP-galactose and flavonols to catalyze the formation of flavonol 3-O-galactosides, F3GalTase catalyzes the reverse reaction, generation of flavonols from UDP and flavonol 3-O-galactosides, almost as efficiently as the forward reaction Petunia x hybrida UDP + flavonol 3-O-beta-D-galactoside
-
r
UDP-galactose + flavonol F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen Petunia x hybrida CMF UDP + flavonol 3-O-beta-D-galactoside
-
?
UDP-galactose + flavonol F3GalTase uses only UDP-galactose and flavonols to catalyze the formation of flavonol 3-O-galactosides, F3GalTase catalyzes the reverse reaction, generation of flavonols from UDP and flavonol 3-O-galactosides, almost as efficiently as the forward reaction Petunia x hybrida CMF UDP + flavonol 3-O-beta-D-galactoside
-
r
UDP-galactose + galangin 15.1% of the activity with kaempferol as substrate Petunia x hybrida UDP + galangin 3-O-beta-D-galactoside
-
r
UDP-galactose + isorhamnetin 13% of the activity with kaempferol as substrate Petunia x hybrida UDP + isorhamnetin 3-O-beta-D-galactoside
-
r
UDP-galactose + kaempferide
-
Petunia x hybrida UDP + kaempferide 3-O-beta-D-galactoside
-
r
UDP-galactose + kaempferol
-
Petunia x hybrida UDP + kaempferol 3-O-beta-D-galactoside
-
r
UDP-galactose + kaempferol
-
Petunia x hybrida CMF UDP + kaempferol 3-O-beta-D-galactoside
-
r
UDP-galactose + morin
-
Petunia x hybrida UDP + morin 3-O-beta-D-galactoside
-
r
UDP-galactose + myricetin 23.4% of the activity with kaempferol as substrate Petunia x hybrida UDP + myricetin 3-O-beta-D-galactoside
-
r
UDP-galactose + quercetin
-
Petunia x hybrida UDP + quercetin 3-O-beta-D-galactoside
-
r
UDP-galactose + quercetin
-
Petunia x hybrida CMF UDP + quercetin 3-O-beta-D-galactoside
-
r
UDP-galactose + rhamnetin 6.9% of the activity with kaempferol as substrate Petunia x hybrida UDP + rhamnetin 3-O-beta-D-galactoside
-
r

Subunits

Subunits Comment Organism
monomer 1 * 45000, native and recombinant F3GalTase, SDS-PAGE, x * 48900, sequence calculation Petunia x hybrida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
assay at, preincubation for 15 min at 43°C results in 5% loss of activity compared with the activity without preincubation Petunia x hybrida
50
-
maximum activity, but preincubation for 15 min at 50°C results in 26% loss of activity compared with the activity without preincubation Petunia x hybrida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
preincubation for 15 min at 43°C results in 5% loss of activity compared with the activity without preincubation Petunia x hybrida
50
-
maximum activity, but preincubation for 15 min at 50°C results in 26% loss of activity compared with the activity without preincubation Petunia x hybrida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50.9
-
UDP pH 6, 43°C, cosubstrate kaempferol 3-O-galactoside, reverse reaction Petunia x hybrida
57.4
-
UDP-galactose pH 7.5, 43°C, cosubstrate quercetin, forward reaction Petunia x hybrida
59.8
-
quercetin pH 7.5, 43°C, forward reaction Petunia x hybrida
59.9
-
kaempferol 3-O-beta-D-galactoside pH 6, 43°C, reverse reaction Petunia x hybrida
61.1
-
kaempferol pH 7.5, 43°C, forward reaction Petunia x hybrida
61.2
-
UDP-galactose pH 7.5, 43°C, cosubstrate kaempferol, forward reaction Petunia x hybrida
82.3
-
quercetin 3-O-galactoside pH 6, 43°C, reverse reaction Petunia x hybrida
89.6
-
UDP pH 6, 43°C, cosubstrate quercetin 3-O-galactoside, reverse reaction Petunia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reverse reaction Petunia x hybrida
7.5 8 forward reaction Petunia x hybrida

pI Value

Organism Comment pI Value Maximum pI Value
Petunia x hybrida
-
-
4.9