Cloned (Comment) | Organism |
---|---|
from pollen, full-length cDNA, expression in Escherichia coli, sequencing | Petunia x hybrida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00096 | - |
quercetin | pH 7.5, 43°C, forward reaction | Petunia x hybrida | |
0.0011 | - |
kaempferol | pH 7.5, 43°C, forward reaction | Petunia x hybrida | |
0.00265 | - |
quercetin 3-O-galactoside | pH 6, 43°C, reverse reaction | Petunia x hybrida | |
0.00847 | - |
kaempferol 3-O-beta-D-galactoside | pH 6, 43°C, reverse reaction | Petunia x hybrida | |
0.066 | - |
UDP | pH 6, 43°C, cosubstrate kaempferol 3-O-galactoside, reverse reaction | Petunia x hybrida | |
0.102 | - |
UDP | pH 6, 43°C, cosubstrate quercetin 3-O-galactoside, reverse reaction | Petunia x hybrida | |
0.387 | - |
UDP-galactose | pH 7.5, 43°C, cosubstrate quercetin, forward reaction | Petunia x hybrida | |
0.426 | - |
UDP-galactose | pH 7.5, 43°C, cosubstrate kaempferol, forward reaction | Petunia x hybrida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant F3GalTase | Petunia x hybrida | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, native and recombinant F3GalTase, SDS-PAGE, x * 48900, sequence calculation | Petunia x hybrida |
48900 | - |
1 * 45000, native and recombinant F3GalTase, SDS-PAGE, x * 48900, sequence calculation | Petunia x hybrida |
50000 | - |
gel filtration, native PAGE | Petunia x hybrida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + flavonol | Petunia x hybrida | F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen | UDP + flavonol 3-O-beta-D-galactoside | - |
? | |
UDP-galactose + flavonol | Petunia x hybrida CMF | F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen | UDP + flavonol 3-O-beta-D-galactoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | Q9SBQ8 | CMF | - |
Petunia x hybrida CMF | Q9SBQ8 | CMF | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | residues 1-30 potentially comprise a cleavable signal sequence | Petunia x hybrida |
Purification (Comment) | Organism |
---|---|
native F3GalTase | Petunia x hybrida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gametophyte | F3GalTase is expressed exclusively in the male gametophyte/pollen | Petunia x hybrida | - |
pollen | from anthers of stage 2-9 flowers, F3GalTase is expressed exclusively in the male gametophyte/pollen, where it modifies a molecule required for functional pollen, expression pattern during development | Petunia x hybrida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: apigenin, naringenin, dihydroquercetin, pelargonidin, delphinidin, cyanidin, NAD+, ferrulic acid, sinapic acid, naphthylphthamic acid, gibberillic acid, indole acetic acid, abscisic acid, jasmonic acid, not in the reverse direction: kaempferol 3-O-glucoside, quercetin 3-O-glucoside | Petunia x hybrida | ? | - |
? | |
additional information | not: apigenin, naringenin, dihydroquercetin, pelargonidin, delphinidin, cyanidin, NAD+, ferrulic acid, sinapic acid, naphthylphthamic acid, gibberillic acid, indole acetic acid, abscisic acid, jasmonic acid, not in the reverse direction: kaempferol 3-O-glucoside, quercetin 3-O-glucoside | Petunia x hybrida CMF | ? | - |
? | |
UDP + kaempferol 3-O-beta-D-galactoside | - |
Petunia x hybrida | UDP-alpha-D-galactose + kaempferol | - |
r | |
UDP-galactose + fisetin | 23.3% of the activity with kaempferol as substrate | Petunia x hybrida | UDP + fisetin 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + flavonol | F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen | Petunia x hybrida | UDP + flavonol 3-O-beta-D-galactoside | - |
? | |
UDP-galactose + flavonol | F3GalTase uses only UDP-galactose and flavonols to catalyze the formation of flavonol 3-O-galactosides, F3GalTase catalyzes the reverse reaction, generation of flavonols from UDP and flavonol 3-O-galactosides, almost as efficiently as the forward reaction | Petunia x hybrida | UDP + flavonol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + flavonol | F3GalTase controls the formation of a pollen-specific class of glycosylated flavonols, role in flavonol-induced pollen germination, F3GalTase is expressed exclusively in the male gametophyte, where it modifies a molecule required for functional pollen | Petunia x hybrida CMF | UDP + flavonol 3-O-beta-D-galactoside | - |
? | |
UDP-galactose + flavonol | F3GalTase uses only UDP-galactose and flavonols to catalyze the formation of flavonol 3-O-galactosides, F3GalTase catalyzes the reverse reaction, generation of flavonols from UDP and flavonol 3-O-galactosides, almost as efficiently as the forward reaction | Petunia x hybrida CMF | UDP + flavonol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + galangin | 15.1% of the activity with kaempferol as substrate | Petunia x hybrida | UDP + galangin 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + isorhamnetin | 13% of the activity with kaempferol as substrate | Petunia x hybrida | UDP + isorhamnetin 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + kaempferide | - |
Petunia x hybrida | UDP + kaempferide 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + kaempferol | - |
Petunia x hybrida | UDP + kaempferol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + kaempferol | - |
Petunia x hybrida CMF | UDP + kaempferol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + morin | - |
Petunia x hybrida | UDP + morin 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + myricetin | 23.4% of the activity with kaempferol as substrate | Petunia x hybrida | UDP + myricetin 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + quercetin | - |
Petunia x hybrida | UDP + quercetin 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + quercetin | - |
Petunia x hybrida CMF | UDP + quercetin 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + rhamnetin | 6.9% of the activity with kaempferol as substrate | Petunia x hybrida | UDP + rhamnetin 3-O-beta-D-galactoside | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, native and recombinant F3GalTase, SDS-PAGE, x * 48900, sequence calculation | Petunia x hybrida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
assay at, preincubation for 15 min at 43°C results in 5% loss of activity compared with the activity without preincubation | Petunia x hybrida |
50 | - |
maximum activity, but preincubation for 15 min at 50°C results in 26% loss of activity compared with the activity without preincubation | Petunia x hybrida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
preincubation for 15 min at 43°C results in 5% loss of activity compared with the activity without preincubation | Petunia x hybrida |
50 | - |
maximum activity, but preincubation for 15 min at 50°C results in 26% loss of activity compared with the activity without preincubation | Petunia x hybrida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50.9 | - |
UDP | pH 6, 43°C, cosubstrate kaempferol 3-O-galactoside, reverse reaction | Petunia x hybrida | |
57.4 | - |
UDP-galactose | pH 7.5, 43°C, cosubstrate quercetin, forward reaction | Petunia x hybrida | |
59.8 | - |
quercetin | pH 7.5, 43°C, forward reaction | Petunia x hybrida | |
59.9 | - |
kaempferol 3-O-beta-D-galactoside | pH 6, 43°C, reverse reaction | Petunia x hybrida | |
61.1 | - |
kaempferol | pH 7.5, 43°C, forward reaction | Petunia x hybrida | |
61.2 | - |
UDP-galactose | pH 7.5, 43°C, cosubstrate kaempferol, forward reaction | Petunia x hybrida | |
82.3 | - |
quercetin 3-O-galactoside | pH 6, 43°C, reverse reaction | Petunia x hybrida | |
89.6 | - |
UDP | pH 6, 43°C, cosubstrate quercetin 3-O-galactoside, reverse reaction | Petunia x hybrida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reverse reaction | Petunia x hybrida |
7.5 | 8 | forward reaction | Petunia x hybrida |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Petunia x hybrida | - |
- |
4.9 |