KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
kaempferol | forward reaction | Petunia sp. | |
0.013 | - |
UDP | cosubstrate kaempferol 3-O-beta-D-galactoside, reverse reaction | Petunia sp. | |
0.039 | - |
kaempferol 3-O-beta-D-galactoside | reverse reaction | Petunia sp. | |
0.318 | - |
UDP-galactose | cosubstrate kaempferol, forward reaction | Petunia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-galactose + quercetin | Petunia sp. | role in pollen germination | UDP + quercetin 3-O-beta-D-galactoside | quercetin 3-O-beta-D-galactoside induces pollen germination via the reverse activity | r | |
UDP-galactose + flavonol | Petunia sp. | F3GalTase controls the formation of glycosylated flavonols in pollen, enzyme also catalzes the reverse reaction, i.e the production of flavonol aglycone from the galactoside and UDP, and has therefore the potential to control the level of the bioactive flavonol species and as a result, pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes, control mechanisms of the reversible reaction | UDP + flavonol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + kaempferol | Petunia sp. | role in pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes | UDP + kaempferol 3-O-beta-D-galactoside | kaempferol 3-O-beta-D-galactoside induces pollen germination via the reverse activity | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Petunia sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen | surface localized | Petunia sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: UDP-glucose | Petunia sp. | ? | - |
? | |
UDP-D-galactose + quercetin | - |
Petunia sp. | UDP + quercetin 3-O-beta-D-galactoside | - |
r | |
UDP-D-galactose + quercetin | role in pollen germination | Petunia sp. | UDP + quercetin 3-O-beta-D-galactoside | quercetin 3-O-beta-D-galactoside induces pollen germination via the reverse activity | r | |
UDP-galactose + flavonol | F3GalTase controls the formation of glycosylated flavonols in pollen, enzyme also catalzes the reverse reaction, i.e the production of flavonol aglycone from the galactoside and UDP, and has therefore the potential to control the level of the bioactive flavonol species and as a result, pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes, control mechanisms of the reversible reaction | Petunia sp. | UDP + flavonol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + flavonol | absolute requirement for UDP-galactose | Petunia sp. | UDP + flavonol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + kaempferol | - |
Petunia sp. | UDP + kaempferol 3-O-beta-D-galactoside | - |
r | |
UDP-galactose + kaempferol | role in pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes | Petunia sp. | UDP + kaempferol 3-O-beta-D-galactoside | kaempferol 3-O-beta-D-galactoside induces pollen germination via the reverse activity | r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reverse reaction | Petunia sp. |
7.5 | - |
forward reaction | Petunia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | the activity of the forward reaction is 1.6fold higher at pH 7.5 than at 5.5, the activity of the reverse reaction is 8fold higher at pH 5.5 than at 7.5 | Petunia sp. |