Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 14 mM, activates | Vigna mungo | |
dithioerythritol | 10 mM, activates | Vigna mungo | |
EDTA | 1-10 mM, activates | Vigna mungo |
General Stability | Organism |
---|---|
14 mM 2-mercaptoethanol stabilizes | Vigna mungo |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM: 29% inhibition, 10 mM: 53% inhibition | Vigna mungo | |
CoCl2 | 1 mM, 62% inhibition | Vigna mungo | |
CuCl2 | 1 mM, 100% inhibition | Vigna mungo | |
iodoacetate | 1 mM, 47% inhibition | Vigna mungo | |
MgCl2 | 1 mM: 31% inhibition, 10 mM: 37% inhibition | Vigna mungo | |
MnCl2 | 1 mM: 62% inhibition, 10 mM: 43% inhibition | Vigna mungo | |
additional information | not inhibited by KCl, iodoacetamide | Vigna mungo | |
N-ethylmaleimide | 1 mM, 89% inhibition, 14 mM 2-mercaptoethanol completely restores activity | Vigna mungo | |
p-chloromercuribenzoate | 1 mM, 100% inhibition, 14 mM 2-mercaptoethanol completely restores activity | Vigna mungo | |
Phenylmercuriacetate | 1 mM, 98% inhibition | Vigna mungo | |
ZnCl2 | 1 mM, 97% inhibition | Vigna mungo |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
kaempferol | pH 8, 30°C | Vigna mungo | |
0.125 | - |
UDP-D-galactose | pH 8, 30°C, cosubstrate kaempferol | Vigna mungo |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Vigna mungo | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Vigna mungo |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna mungo | - |
NIAS no. R-30 | - |
Purification (Comment) | Organism |
---|---|
404.3fold | Vigna mungo |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-galactose + kaempferol = UDP + kaempferol 3-O-beta-D-galactoside | acts on the endogenous flavonols kaempferol and quercetin, to a lesser extend on myricetin and fisetin, and weakly on galangin and isorhamnetin, the reaction can occur equally well in both directions | Vigna mungo |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | 8 days old | Vigna mungo | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.335 | - |
pH 8, 30°C | Vigna mungo |
Storage Stability | Organism |
---|---|
-20°C, 20 mM imidazole-HCl, pH 8, 10% glycerol, one week, 100% loss of activity | Vigna mungo |
-20°C, 20 mM imidazole-HCl, pH 8, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days or one week, 35-36% loss of activity | Vigna mungo |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no/poor substrates: kaempferol 5,7,4-trimethylether, quercetin 5-monomethylether, kaempferol 3-O-galactoside, kaempferol 7-O-glucoside, kaempferol 7-O-rhamnoside, dihydrokaempferol, dihydroquercetin, eriodictyol | Vigna mungo | ? | - |
? | |
UDP-D-galactose + isorhamnetin | kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate, 106% of the activity with kaempferol as substrate | Vigna mungo | UDP + isorhamnetin 3-O-beta-D-galactoside | - |
? | |
UDP-D-galactose + kaempferol | kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate | Vigna mungo | UDP + kaempferol 3-O-beta-D-galactoside | trifolin | ? | |
UDP-D-galactose + kaempferol 4'-monomethylether | 79% of the activity with kaempferol as substrate | Vigna mungo | UDP + kaempferol 4'-monomethylether 3-O-beta-D-galactoside | - |
? | |
UDP-D-galactose + myricetin | 28% of the activity with kaempferol as substrate | Vigna mungo | UDP + myricetin 3-O-beta-D-galactoside | - |
? | |
UDP-D-galactose + quercetin | kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate, 104% of the activity with kaempferol as substrate | Vigna mungo | UDP + quercetin 3-O-beta-D-galactoside | - |
? | |
UDP-D-galactose + quercetin 7-O-glucoside | 33% of the activity with kaempferol as substrate | Vigna mungo | UDP + quercetin 7-O-glucoside-3-O-beta-D-galactoside | - |
? | |
UDP-D-galactose + rhamnetin | 75% of the activity with kaempferol as substrate | Vigna mungo | UDP + rhamnetin 3-O-beta-D-galactoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3GalTase | - |
Vigna mungo |
F3GaT | - |
Vigna mungo |
flavonoid 3-O-galactosyltransferase | - |
Vigna mungo |
flavonol 3-O-galactosyltransferase | - |
Vigna mungo |
galactosyltransferase, uridine diphosphogalactose-flavonol 3-O- | - |
Vigna mungo |
UDP-galactose:flavonoid 3-O-galactosyltransferase | - |
Vigna mungo |
UDP-galactose:flavonol 3-O-galactosyltransferase | - |
Vigna mungo |
UDPgalactose-flavonoid-3-O-glycosyltransferase | - |
Vigna mungo |
uridine diphosphogalactose-flavonol 3-O-galactosyltransferase | - |
Vigna mungo |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in histidine-HCl buffer | Vigna mungo |
8 | - |
in imidazole-HCl buffer | Vigna mungo |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vigna mungo | chromatofocusing | - |
6 |