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Literature summary for 2.4.1.234 extracted from

  • Ishikura, N.; Mato, M.
    Partial purification and some properties of flavonol 3-O-glycosyltransferases from seedlings of Vigna mungo, with special reference to the formation of kaempferol 3-O-galactoside and 3-O-glucoside (1993), Plant Cell Physiol., 34, 329-335.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 14 mM, activates Vigna mungo
dithioerythritol 10 mM, activates Vigna mungo
EDTA 1-10 mM, activates Vigna mungo

General Stability

General Stability Organism
14 mM 2-mercaptoethanol stabilizes Vigna mungo

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 1 mM: 29% inhibition, 10 mM: 53% inhibition Vigna mungo
CoCl2 1 mM, 62% inhibition Vigna mungo
CuCl2 1 mM, 100% inhibition Vigna mungo
iodoacetate 1 mM, 47% inhibition Vigna mungo
MgCl2 1 mM: 31% inhibition, 10 mM: 37% inhibition Vigna mungo
MnCl2 1 mM: 62% inhibition, 10 mM: 43% inhibition Vigna mungo
additional information not inhibited by KCl, iodoacetamide Vigna mungo
N-ethylmaleimide 1 mM, 89% inhibition, 14 mM 2-mercaptoethanol completely restores activity Vigna mungo
p-chloromercuribenzoate 1 mM, 100% inhibition, 14 mM 2-mercaptoethanol completely restores activity Vigna mungo
Phenylmercuriacetate 1 mM, 98% inhibition Vigna mungo
ZnCl2 1 mM, 97% inhibition Vigna mungo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
kaempferol pH 8, 30°C Vigna mungo
0.125
-
UDP-D-galactose pH 8, 30°C, cosubstrate kaempferol Vigna mungo

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Vigna mungo
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
gel filtration Vigna mungo

Organism

Organism UniProt Comment Textmining
Vigna mungo
-
NIAS no. R-30
-

Purification (Commentary)

Purification (Comment) Organism
404.3fold Vigna mungo

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-galactose + kaempferol = UDP + kaempferol 3-O-beta-D-galactoside acts on the endogenous flavonols kaempferol and quercetin, to a lesser extend on myricetin and fisetin, and weakly on galangin and isorhamnetin, the reaction can occur equally well in both directions Vigna mungo

Source Tissue

Source Tissue Comment Organism Textmining
seedling 8 days old Vigna mungo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.335
-
pH 8, 30°C Vigna mungo

Storage Stability

Storage Stability Organism
-20°C, 20 mM imidazole-HCl, pH 8, 10% glycerol, one week, 100% loss of activity Vigna mungo
-20°C, 20 mM imidazole-HCl, pH 8, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days or one week, 35-36% loss of activity Vigna mungo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no/poor substrates: kaempferol 5,7,4’-trimethylether, quercetin 5-monomethylether, kaempferol 3-O-galactoside, kaempferol 7-O-glucoside, kaempferol 7-O-rhamnoside, dihydrokaempferol, dihydroquercetin, eriodictyol Vigna mungo ?
-
?
UDP-D-galactose + isorhamnetin kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate, 106% of the activity with kaempferol as substrate Vigna mungo UDP + isorhamnetin 3-O-beta-D-galactoside
-
?
UDP-D-galactose + kaempferol kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate Vigna mungo UDP + kaempferol 3-O-beta-D-galactoside trifolin ?
UDP-D-galactose + kaempferol 4'-monomethylether 79% of the activity with kaempferol as substrate Vigna mungo UDP + kaempferol 4'-monomethylether 3-O-beta-D-galactoside
-
?
UDP-D-galactose + myricetin 28% of the activity with kaempferol as substrate Vigna mungo UDP + myricetin 3-O-beta-D-galactoside
-
?
UDP-D-galactose + quercetin kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate, 104% of the activity with kaempferol as substrate Vigna mungo UDP + quercetin 3-O-beta-D-galactoside
-
?
UDP-D-galactose + quercetin 7-O-glucoside 33% of the activity with kaempferol as substrate Vigna mungo UDP + quercetin 7-O-glucoside-3-O-beta-D-galactoside
-
?
UDP-D-galactose + rhamnetin 75% of the activity with kaempferol as substrate Vigna mungo UDP + rhamnetin 3-O-beta-D-galactoside
-
?

Synonyms

Synonyms Comment Organism
F3GalTase
-
Vigna mungo
F3GaT
-
Vigna mungo
flavonoid 3-O-galactosyltransferase
-
Vigna mungo
flavonol 3-O-galactosyltransferase
-
Vigna mungo
galactosyltransferase, uridine diphosphogalactose-flavonol 3-O-
-
Vigna mungo
UDP-galactose:flavonoid 3-O-galactosyltransferase
-
Vigna mungo
UDP-galactose:flavonol 3-O-galactosyltransferase
-
Vigna mungo
UDPgalactose-flavonoid-3-O-glycosyltransferase
-
Vigna mungo
uridine diphosphogalactose-flavonol 3-O-galactosyltransferase
-
Vigna mungo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
in histidine-HCl buffer Vigna mungo
8
-
in imidazole-HCl buffer Vigna mungo

pI Value

Organism Comment pI Value Maximum pI Value
Vigna mungo chromatofocusing
-
6