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Literature summary for 2.4.1.231 extracted from

  • Goedl, C.; Griessler, R.; Schwarz, A.; Nidetzky, B.
    Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases (2006), Biochem. J., 397, 491-500.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.231

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Schizophyllum commune

Protein Variants

Protein Variants Comment Organism
D379N ratio of phosphorolysis to hydrolysis decreases by a factor of 287500 Schizophyllum commune
H403A ratio of phosphorolysis to hydrolysis decreases by a factor of 2190 Schizophyllum commune
K512A ratio of phosphorolysis to hydrolysis decreases by a factor of 511111 Schizophyllum commune
R507A ratio of phosphorolysis to hydrolysis decreases by a factor of 575000 Schizophyllum commune

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-glucose 1-phosphate
-
 Schizophyllum commune
phosphate
-
 Schizophyllum commune
validoxylamine A
-
 Schizophyllum commune

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
1.4
-
 phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
1.6
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
2.2
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
35
-
 alpha-D-glucose 30°C, pH 6.6, natural enzyme Schizophyllum commune
46
-
 alpha-D-glucose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
53
-
 alpha,alpha-trehalose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
71
-
 alpha,alpha-trehalose 30°C, pH 6.6, natural enzyme Schizophyllum commune

Organism

Organism UniProt Comment Textmining
Schizophyllum commune Q2HZZ3 BT 2115
-
Schizophyllum commune BT 2115 Q2HZZ3 BT 2115
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Schizophyllum commune

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose + alpha-D-glucose 1-phosphate ratio of phosphorolysis to hydrolysis is 46000 Schizophyllum commune alpha,alpha-trehalose + phosphate
-
 r
alpha-D-glucose + alpha-D-glucose 1-phosphate ratio of phosphorolysis to hydrolysis is 46000 Schizophyllum commune BT 2115 alpha,alpha-trehalose + phosphate
-
 r

Synonyms

Synonyms Comment Organism
ScTPase
-
 Schizophyllum commune

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.5
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
9.5
-
 alpha-D-glucose 30°C, pH 6.6, natural enzyme Schizophyllum commune
13.3
-
 phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
13.3
-
 alpha,alpha-trehalose 30°C, pH 6.6, natural enzyme Schizophyllum commune
14.1
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
14.1
-
 alpha-D-glucose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
16.4
-
 phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
16.4
-
 alpha,alpha-trehalose 30°C, pH 6.6, recombinant enzyme Schizophyllum commune

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0017
-
 validoxylamine A
-
 Schizophyllum commune
5.8
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune
5.9
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
12
-
 phosphate 30°C, pH 6.6, recombinant enzyme Schizophyllum commune
26
-
 phosphate 30°C, pH 6.6, natural enzyme Schizophyllum commune