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Literature summary for 2.4.1.231 extracted from

  • Wannet, W.J.; Op den Camp, H.J.; Wisselink, H.W.; van der Drift, C.; Van Griensven, L.J.; Vogels, G.D.
    Purification and characterization of trehalose phosphorylase from the commercial mushroom Agaricus bisporus (1998), Biochim. Biophys. Acta, 1425, 177-188.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
AMP stimulates phosphorolytic activity at 10 mM Agaricus bisporus
ATP stimulates synthetic activity at 10 mM Agaricus bisporus

General Stability

General Stability Organism
highly unstable during purification, glycerol, phosphate, dithiothreitol and PMSF stabilize Agaricus bisporus

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin 88% inhibition at 10 mM Agaricus bisporus
ADP 34% inhibition at 10 mM Agaricus bisporus
ATP inhibits trehalose degradation, but stimulates synthetic activity Agaricus bisporus
CoCl2 39% inhibition at 1 mM Agaricus bisporus
CuSO4 37% inhibition at 1 mM Agaricus bisporus
phosphate inhibits synthetic activity Agaricus bisporus
validamycin A 99% inhibition at 10 mM Agaricus bisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.7
-
phosphate 30°C, pH 7 Agaricus bisporus
6.3
-
alpha-D-glucose 1-phosphate 30°C, pH 7 Agaricus bisporus
24
-
alpha-D-glucose 30°C, pH 7 Agaricus bisporus
61
-
alpha,alpha-trehalose 30°C, pH 7 Agaricus bisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
4 * 61000, SDS-PAGE Agaricus bisporus
240000
-
native PAGE, gel filtration Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + phosphate Agaricus bisporus key role in trehalose metabolism alpha-D-glucose + alpha-D-glucose 1-phosphate
-
r
alpha,alpha-trehalose + phosphate Agaricus bisporus Horst U1 key role in trehalose metabolism alpha-D-glucose + alpha-D-glucose 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-
Agaricus bisporus Horst U1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Agaricus bisporus

Reaction

Reaction Comment Organism Reaction ID
alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate mechanism Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Agaricus bisporus
-
mycelium
-
Agaricus bisporus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.35
-
-
Agaricus bisporus

Storage Stability

Storage Stability Organism
-80°C, stable for at least 6 months Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + phosphate
-
Agaricus bisporus alpha-D-glucose + alpha-D-glucose 1-phosphate
-
r
alpha,alpha-trehalose + phosphate key role in trehalose metabolism Agaricus bisporus alpha-D-glucose + alpha-D-glucose 1-phosphate
-
r
alpha,alpha-trehalose + phosphate
-
Agaricus bisporus Horst U1 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
r
alpha,alpha-trehalose + phosphate key role in trehalose metabolism Agaricus bisporus Horst U1 alpha-D-glucose + alpha-D-glucose 1-phosphate
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 61000, SDS-PAGE Agaricus bisporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
almost complete loss of activity after 60 min Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 synthesis of trehalose Agaricus bisporus
6 7.5 phosphorolytic reaction Agaricus bisporus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
phosphate 30°C, pH 7 Agaricus bisporus

pI Value

Organism Comment pI Value Maximum pI Value
Agaricus bisporus isoelectric focusing between pH 3-9 and pH 3-10 4.8 4.7