BRENDA - Enzyme Database show
show all sequences of 2.4.1.228

alpha-1,4Galactosyltransferase activity and Gbeta3Cer expression in human leukemia/lymphoma cell lines

Stults, C.L.M.; Larsen, R.D.; Macher, B.A.; Glycoconj. J. 12, 680-689 (1995)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
lactosylceramide
-
Homo sapiens
0.52
-
UDP-galactose
-
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
THP-1 cells
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide
Homo sapiens
-
UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
leukaemia-myeloid cell lines: K562, KG-1, HL-60, and THP-1, lymphoma-lymphoid cell lines: Reh, Daudi, Raji, RPMI 8226, CCRF-CEM, and MOLT-4
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
DAUDI cell
-
Homo sapiens
-
additional information
THP-1, RPMI 8226, Daudi, and Raji cell lines with detectable enzyme activity, no detectable enzyme activity: K562, KG-1, HL-60, Reh, MOLT-4, CCRF-CEM cell lines
Homo sapiens
-
RAJI cell
-
Homo sapiens
-
RPMI-8226 cell
-
Homo sapiens
-
THP-1 cell
-
Homo sapiens
-
Storage Stability
Storage Stability
Organism
-70C, however enzyme activity in RPMI 8226 cells destroyed by storage at -70C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide
-
488983
Homo sapiens
UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide
-
488983
Homo sapiens
?
UDP-galactose + lactosylceramide
-
488983
Homo sapiens
UDP + globotriaosylceramide
-
488983
Homo sapiens
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
THP-1 cells
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
lactosylceramide
-
Homo sapiens
0.52
-
UDP-galactose
-
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
THP-1 cells
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide
Homo sapiens
-
UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide
-
Homo sapiens
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
DAUDI cell
-
Homo sapiens
-
additional information
THP-1, RPMI 8226, Daudi, and Raji cell lines with detectable enzyme activity, no detectable enzyme activity: K562, KG-1, HL-60, Reh, MOLT-4, CCRF-CEM cell lines
Homo sapiens
-
RAJI cell
-
Homo sapiens
-
RPMI-8226 cell
-
Homo sapiens
-
THP-1 cell
-
Homo sapiens
-
Storage Stability (protein specific)
Storage Stability
Organism
-70C, however enzyme activity in RPMI 8226 cells destroyed by storage at -70C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide
-
488983
Homo sapiens
UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide
-
488983
Homo sapiens
?
UDP-galactose + lactosylceramide
-
488983
Homo sapiens
UDP + globotriaosylceramide
-
488983
Homo sapiens
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
THP-1 cells
Homo sapiens
Other publictions for EC 2.4.1.228
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735618
Kaczmarek
Human Gb3/CD77 synthase reveal ...
Homo sapiens
Biochem. Biophys. Res. Commun.
470
168-174
2016
-
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1
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1
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1
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1
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1
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1
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1
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4
1
1
-
-
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1
-
-
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1
1
-
-
-
736066
Tyler
Targeting glucosylceramide syn ...
Homo sapiens
Exp. Cell Res.
336
23-32
2015
1
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1
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2
2
-
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722791
Suchanowska
A single point mutation in the ...
Homo sapiens
J. Biol. Chem.
287
38220-38230
2012
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1
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2
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2
2
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722567
Shiozuka
Increased globotriaosylceramid ...
Homo sapiens
J. Biochem.
149
161-170
2011
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-
1
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1
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2
2
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722691
Yamaji
Transmembrane BAX inhibitor mo ...
Homo sapiens
J. Biol. Chem.
285
35505-35518
2010
-
-
1
-
-
-
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1
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1
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3
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2
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1
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1
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1
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2
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1
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2
2
-
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-
702866
Shin
Globotriaosylceramide (Gb3) co ...
Homo sapiens
BMB Rep.
42
310-314
2009
-
-
-
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2
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1
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703661
Okuda
A novel, promoter-based, targe ...
Homo sapiens
FEBS J.
276
5191-5202
2009
-
-
-
-
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-
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1
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1
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2
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4
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1
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1
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1
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4
-
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1
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1
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-
1
-
-
703901
Ramkumar
Induction of HIV-1 resistance: ...
Homo sapiens
Glycobiology
19
76-82
2009
-
-
-
-
-
-
1
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1
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2
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2
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1
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1
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-
692526
Okuda
Identification and characteriz ...
Homo sapiens
Glycobiology
18
1028-1035
2008
3
1
1
-
-
-
3
-
-
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1
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4
-
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5
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1
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3
1
1
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3
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1
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5
-
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1
-
-
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-
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-
-
-
-
-
-
-
-
692534
Schweppe
Glycosphingolipids in vascular ...
Homo sapiens
Glycoconj. J.
25
291-304
2008
-
1
-
-
-
-
-
-
-
-
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1
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2
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4
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1
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1
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1
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4
-
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1
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674524
Okuda
Targeted disruption of Gb3/CD7 ...
Mus musculus
J. Biol. Chem.
281
10230-10235
2006
-
-
-
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-
-
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1
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3
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4
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4
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1
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-
659273
Iwamura
The blood group P1 synthase ge ...
Homo sapiens
J. Biol. Chem.
278
44429-44438
2003
-
-
1
-
-
-
-
-
2
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2
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1
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1
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3
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1
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2
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1
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3
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488985
Kojima
Molecular cloning of globotria ...
Homo sapiens
J. Biol. Chem.
275
15152-15156
2000
-
-
1
-
-
-
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2
-
1
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1
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2
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1
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3
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1
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1
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1
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1
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3
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488986
Steffensen
Cloning and expression of the ...
Homo sapiens
J. Biol. Chem.
275
16723-16729
2000
-
-
1
-
-
-
-
-
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2
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1
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1
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1
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488983
Stults
alpha-1,4Galactosyltransferase ...
Homo sapiens
Glycoconj. J.
12
680-689
1995
-
-
-
-
-
-
-
2
-
1
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1
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1
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5
-
1
2
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1
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2
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1
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1
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5
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1
2
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1
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488984
Mobassaleh
Developmentally regulated Gb3 ...
Oryctolagus cuniculus
Am. J. Physiol.
267
G618-G624
1994
-
-
-
-
-
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-
1
2
1
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1
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2
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4
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1
4
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1
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488982
Chen
Glycolipid glycosyltransferase ...
Homo sapiens
Biochemistry
28
2229-2238
1989
-
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488980
Bailly
Identification of UDP-galactos ...
Homo sapiens
Biochem. Biophys. Res. Commun.
141
84-91
1986
-
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3
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488981
Iizuka
Studies on the human blood gro ...
Homo sapiens
Biochem. Biophys. Res. Commun.
137
1187-1195
1986
-
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1
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2
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1
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488979
Taniguchi
Purification and properties of ...
Rattus norvegicus
J. Biol. Chem.
260
4908-4913
1985
-
-
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2
-
3
3
1
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2
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1
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2
1
1
4
1
1
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1
1
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3
1
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1
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1
1
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1
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