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Literature summary for 2.4.1.228 extracted from
- Purification and properties of rat liver globotriaosylceramide synthase, UDP-galactose:lactosylceramide alpha1-4-galactosyltransferase (1985), J. Biol. Chem., 260, 4908-4913.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
lactosylceramide | - |
Rattus norvegicus |  |
| 0.03 | - |
UDP-galactose | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | some activity | Rattus norvegicus | |
| Mn2+ | a second divalent cation in the enzyme assay can inhibit the enzyme activity | Rattus norvegicus | |
| Ni2+ | some activity | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE | Rattus norvegicus |
| 65000 | - |
alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE | Rattus norvegicus |
| 90000 | - |
corresponding to the Stokes radius of 40 A, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide | Rattus norvegicus | - |
UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | stored at -80°C until use | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00458 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 20% glycerol, stored at a concentration greater than 0.1 mg/ml without detectable loss of activity upon repeated freezing and thawing for 3 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrates: galactosylceramide, glucosylceramide, neolactotetraose, neolactotetraosylceramide | Rattus norvegicus | ? | - |
? | |
| UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide | - |
Rattus norvegicus | UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide | - |
? | |
| UDP-galactose + lactose | - |
Rattus norvegicus | ? | - |
? | |
| UDP-galactose + lactosylceramide | UDP-galactose is the sole sugar donor | Rattus norvegicus | UDP + globotriaosylceramide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.4 | - |
Rattus norvegicus |
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