Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli cells BL21-DE3-pLysS | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | competitive inhibitor of UDP-GlcNAc donor, noncompetitive inhibitor of lipid I acceptor analogue | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
synthetic substrate analogue 7 | derivative containing a 20 carbon chain with a cis-allylic double bond | Escherichia coli | |
0.553 | - |
synthetic substrate analogue 2 | 10 carbon citronellyl derivative | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | MurG utilizes an ordered Bi Bi mechanism in which the donor binds first | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MurG prefers substrates that contain lipid chains that are considerably shorter than that of the natural substrate, compounds that contain a cis-allylic double bond react faster than compounds that do not | Escherichia coli | ? | - |
? | |
UDP-N-acetylglucosamine + synthetic substrate analogue 2 | synthetic substrate analogue 2: a 10 carbon citronellyl derivative | Escherichia coli | ? | - |
? | |
UDP-N-acetylglucosamine + synthetic substrate analogue 7 | synthetic substrate analogue 7: a derivative containing a 20 carbon chain with a cis-allylic double bond | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.23 | - |
synthetic substrate analogue 2 | 10 carbon citronellyl derivative | Escherichia coli | |
14 | - |
synthetic substrate analogue 7 | derivative containing a 20 carbon chain with a cis-allylic double bond | Escherichia coli |