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Literature summary for 2.4.1.227 extracted from

  • Chen, L.; Men, H.; Ha, S.; Ye, X.Y.; Brunner, L.; Hu, Y.; Walker, S.
    Intrinsic lipid preferences and kinetic mechanism of Escherichia coli MuG (2002), Biochemistry, 41, 6824-6833.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Mg2+
-
Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli cells BL21-DE3-pLysS Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
UDP competitive inhibitor of UDP-GlcNAc donor, noncompetitive inhibitor of lipid I acceptor analogue Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
synthetic substrate analogue 7 derivative containing a 20 carbon chain with a cis-allylic double bond Escherichia coli
0.553
-
synthetic substrate analogue 2 10 carbon citronellyl derivative Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol MurG utilizes an ordered Bi Bi mechanism in which the donor binds first Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MurG prefers substrates that contain lipid chains that are considerably shorter than that of the natural substrate, compounds that contain a cis-allylic double bond react faster than compounds that do not Escherichia coli ?
-
?
UDP-N-acetylglucosamine + synthetic substrate analogue 2 synthetic substrate analogue 2: a 10 carbon citronellyl derivative Escherichia coli ?
-
?
UDP-N-acetylglucosamine + synthetic substrate analogue 7 synthetic substrate analogue 7: a derivative containing a 20 carbon chain with a cis-allylic double bond Escherichia coli ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.23
-
synthetic substrate analogue 2 10 carbon citronellyl derivative Escherichia coli
14
-
synthetic substrate analogue 7 derivative containing a 20 carbon chain with a cis-allylic double bond Escherichia coli