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Literature summary for 2.4.1.220 extracted from

  • Marcinek, H.; Weyler, W.; Deus-Neumann, B.; Zenk, M.H.
    Indoxyl-UDPG-glucosyltransferase from Baphicacanthus cusia (2000), Phytochemistry, 53, 201-207.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
5-hydroxyindole
-
Strobilanthes cusia
1.2
-
indoxyl
-
Strobilanthes cusia
1.4
-
7-hydroxyindole
-
Strobilanthes cusia
1.7
-
UDP-glucose
-
Strobilanthes cusia
1.9
-
4-hydroxyindole
-
Strobilanthes cusia
3.3
-
6-hydroxyindole
-
Strobilanthes cusia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Strobilanthes cusia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + indoxyl Strobilanthes cusia indole is the biosynthetic precursor to the indoxyl derivates indican isatan B UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?

Organism

Organism UniProt Comment Textmining
Strobilanthes cusia
-
-
-

Oxidation Stability

Oxidation Stability Organism
anaerobic conditions, since under aerobic conditions indoxyl dimerizes spontaneously to indigo Strobilanthes cusia

Purification (Commentary)

Purification (Comment) Organism
-
Strobilanthes cusia

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Strobilanthes cusia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Strobilanthes cusia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: 2-OH-indole Strobilanthes cusia ?
-
?
UDP-glucose + 4-hydroxyindole minimal activity Strobilanthes cusia UDP + 1H-indol-4-yl-beta-D-glucopyranoside
-
?
UDP-glucose + 5-hydroxyindole activity as 3-OH-indole Strobilanthes cusia UDP + 1H-indol-5-yl-beta-D-glucopyranoside
-
?
UDP-glucose + 6-hydroxyindole minimal activity Strobilanthes cusia UDP + 1H-indol-6-yl-beta-D-glucopyranoside
-
?
UDP-glucose + 7-hydroxyindole
-
Strobilanthes cusia UDP + 1H-indol-7-yl-beta-D-glucopyranoside
-
?
UDP-glucose + indoxyl best substrate Strobilanthes cusia UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?
UDP-glucose + indoxyl i.e. indole-3-ol or 3-OH-indole Strobilanthes cusia UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?
UDP-glucose + indoxyl indole is the biosynthetic precursor to the indoxyl derivates indican isatan B Strobilanthes cusia UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Strobilanthes cusia
45 48
-
Strobilanthes cusia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Strobilanthes cusia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
pI: 6.5 Strobilanthes cusia

pH Range

pH Minimum pH Maximum Comment Organism
6.8 10.2 6.8 and 10.2 half-maximal-activity Strobilanthes cusia