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Literature summary for 2.4.1.22 extracted from
- Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the beta1,4-galactosyltransferase-I (2001), J. Mol. Biol., 310, 205-218.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| catalytic domain of recombinant bovine beta4Gal-T1 from residues 130 to 402, d129 beta4Gal-T1, 33000 Da, and mouse recombinant alpha-lactalbumin. Crystal structure of enzyme bound with various substrates | Mus musculus |
| catalytic domain of recombinant bovine beta4Gal-T1 from residues 130 to 402, d129 beta4Gal-T1, 33000 Da, and mouse recombinant alpha-lactalbumin. Crystal structure of enzyme bound with various substrates | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
catalytic domain of recombinant bovine beta4Gal-T1 from residues 130 to 402, d129 beta4Gal-T1, 33000 Da, and mouse recombinant alpha-lactalbumin | - |
| Mus musculus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | upon substrate binding to beta4Gal-T1, a large conformational change occurs in the region comprising residues 345 to 365. The role of alpha-lactalbumin is to hold glucose by hydrogen bonding with the O-1 hydroxyl group in the acceptor-binding site on beta4Gal-T1, while the N-acetyl group-binding pocket in beta4Gal-T1 adjusts to maximize the interactions with the glucose molecule | Mus musculus | ? | - |
? |  |
| additional information | upon substrate binding to beta4Gal-T1, a large conformational change occurs in the region comprising residues 345 to 365. The role of alpha-lactalbumin is to hold glucose by hydrogen bonding with the O-1 hydroxyl group in the acceptor-binding site on beta4Gal-T1, while the N-acetyl group-binding pocket in beta4Gal-T1 adjusts to maximize the interactions with the glucose molecule | Bos taurus | ? | - |
? | |
| UDPgalactose + D-glucose | - |
Mus musculus | lactose + UDP | - |
? | |
| UDPgalactose + D-glucose | - |
Bos taurus | lactose + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Bos taurus |
| More | the enzyme is 1.1 complex of a catalytic component beta1,4-galactosyltransferase (beta4gal-T1) and a regulatory component, alpha-lactalbumin | Mus musculus |
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