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Literature summary for 2.4.1.219 extracted from

  • Ruyter, C.M.; Stoeckigt, J.H.H.
    Enzymatic formation of Raucaffricine, the major indole alkaloid of Rauwolfia serpentina cell-suspension cultures (1991), Helv. Chim. Acta, 74, 1707-1712.
No PubMed abstract available
Search for more literature for 2.4.1.219

General Stability

General Stability Organism
2-mercaptoethanol stabilizes transferase activity Rauvolfia serpentina
ascorbic acid stabilizes transferase activity Rauvolfia serpentina
dithioerythritol stabilizes transferase activity Rauvolfia serpentina

Inhibitors

Inhibitors Comment Organism Structure
(phenylmethyl)sulfonyl fluoride strong inhibition at 10 mM Rauvolfia serpentina
1,10-phenanthroline strong inhibition at 5 mM Rauvolfia serpentina
5,5'-dithiobis(2-nitrobenzoic acid) strong inhibition at 1.25 mM Rauvolfia serpentina
Cu2+ complete inhibition at 10 mM Rauvolfia serpentina
EDTA strong inhibition at 5 mM Rauvolfia serpentina
Fe3+ complete inhibition at 10 mM Rauvolfia serpentina
Hg2+ complete inhibition at 10 mM Rauvolfia serpentina
p-Hydroxybenzaldehyde strong inhibition at 5 mM Rauvolfia serpentina
Zn2+ complete inhibition at 10 mM Rauvolfia serpentina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 Vomilenine
-
 Rauvolfia serpentina
0.8
-
 UDP-glucose
-
 Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + vomilenine Rauvolfia serpentina
-
 UDP + raucaffricine
-
 ?

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rauvolfia serpentina

Storage Stability

Storage Stability Organism
-20°C, 3 weeks, no loss in activity Rauvolfia serpentina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + vomilenine
-
 Rauvolfia serpentina UDP + raucaffricine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rauvolfia serpentina