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Literature summary for 2.4.1.217 extracted from
- Crystallization and preliminary X-ray analysis of mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27 (2009), Acta Crystallogr. Sect. F, 65, 1014-1017.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Rosetta cells | Thermus thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 0.2 M magnesium acetate, 0.1 M sodium cacodylate pH 6.5, 30-35% MPD with 600 mM ZnCl2, the addition of Zn2+ to the crystallization buffer is essential in order to obtain crystals | Thermus thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43600 | - |
x * 43600, SDS-PAGE | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + 3-phospho-D-glycerate | Thermus thermophilus | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? |  |
| GDP-mannose + 3-phospho-D-glycerate | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography, Resource Q column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + 3-phospho-D-glycerate | - |
Thermus thermophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43600, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mannosyl-3-phosphoglycerate synthase | - |
Thermus thermophilus |
| MPGS | - |
Thermus thermophilus |
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