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Literature summary for 2.4.1.217 extracted from
- Specialized roles of the two pathways for the synthesis of mannosylglycerate in osmoadaptation and thermoadaptation of Rhodothermus marinus (2004), J. Biol. Chem., 279, 9892-9898.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the isozyme involved in the two-step pathway is upregulated by osmotic stress, the isozyme involved in the one-step pathway is upregulated by heat stress | Rhodothermus marinus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mpgs, DNA and amino acid sequence determination and analysis of the 2 isozymes, expression in Escherichia coli strain BL21(DE) | Rhodothermus marinus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP | 50% inhibition at 2.5 mM | Rhodothermus marinus |  |
| KCl | - |
Rhodothermus marinus | |
| additional information | no inhibition by 2-(alpha-D-mannosyl)-3-phosphoglycerate or mannosylglycerate | Rhodothermus marinus | |
| NaCl | - |
Rhodothermus marinus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Rhodothermus marinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45584 | - |
x * 48795, isozyme 1, mass spectrometry, x * 45584, isozyme 2, mass spectrometry | Rhodothermus marinus |
| 48795 | - |
x * 48795, isozyme 1, mass spectrometry, x * 45584, isozyme 2, mass spectrometry | Rhodothermus marinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + 3-phospho-D-glycerate | Rhodothermus marinus | 2 pathways for mannosylglycerate biosynthesis, a one-step and a two-step pathway, with specialized roles in osmoadaptation and thermoadaptation, the isozyme involved in the two-step pathway is upregulated by osmotic stress, the isozyme involved in the one-step pathway is upregulated by heat stress, regulatory mechanisms, overview | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| additional information | Rhodothermus marinus | the 30 amino acid C-terminal sequence of the enzyme has regulatory function, cleaving of this peptide increases the activity by 10fold | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodothermus marinus | Q6WSQ4 | gene mpgs, 2 isozymes | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the 30 amino acid C-terminal sequence of the enzyme has regulatory function, cleaving of this peptide by intracellular proteases increases the activity by 10fold | Rhodothermus marinus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozymes in a multistep procedure, recombinant isozymes from Escherichia coli by 3 chromatographic steps of cation exchange chromatography and gel filtration | Rhodothermus marinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156 | - |
purified enzyme lacking the 30 amino acids of the C-terminus | Rhodothermus marinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + 3-phospho-D-glycerate | - |
Rhodothermus marinus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | 2 pathways for mannosylglycerate biosynthesis, a one-step and a two-step pathway, with specialized roles in osmoadaptation and thermoadaptation, the isozyme involved in the two-step pathway is upregulated by osmotic stress, the isozyme involved in the one-step pathway is upregulated by heat stress, regulatory mechanisms, overview | Rhodothermus marinus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| additional information | the 30 amino acid C-terminal sequence of the enzyme has regulatory function, cleaving of this peptide increases the activity by 10fold | Rhodothermus marinus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48795, isozyme 1, mass spectrometry, x * 45584, isozyme 2, mass spectrometry | Rhodothermus marinus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MPGS | - |
Rhodothermus marinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Rhodothermus marinus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 95 | temperature-profile of full length and truncated enzyme | Rhodothermus marinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life truncated enzyme: 79 min, half-life full length enzyme: 40 min | Rhodothermus marinus |
| 100 | - |
85% remaining activity of the truncated enzyme, the full length enzyme is inactive | Rhodothermus marinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Rhodothermus marinus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | pH-profile of full length and truncated enzyme | Rhodothermus marinus |
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