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Literature summary for 2.4.1.216 extracted from

  • Taguchi, Y.; Saburi, W.; Imai, R.; Mori, H.
    Efficient one-pot enzymatic synthesis of trehalose 6-phosphate using GH65 alpha-glucoside phosphorylases (2020), Carbohydr. Res., 488, 107902 .
    View publication on PubMed

Application

Application Comment Organism
synthesis efficient one-pot enzymatic synthesis of trehalose 6-phosphate. The one-pot reaction of trehalose 6-phosphate phosphorylase and maltose phosphorylase enables production of 65 mM trehalose 6-phosphate from 100 mM maltose, 100 mM Glc6P, and 20 mM inorganic phosphate. Addition of beta-phosphoglucomutase to this reaction produces glucose 6-phosphate from beta-glucose 1-phosphate and thus reduces requirement of glucose 6-phosphate as a starting material. Within the range of 20-469 mM inorganic phosphate tested, the 54 mM concentration yielded the highest amount of trehalose 6-phosphate (33 mM). Addition of yeast increases the yield because of its glucose consumption. From 100 mmol maltose and 60 mmol inorganic phosphate, the production of 37.5 mmol trehalose 6-phosphate in a one-pot reaction (100 ml) is achieved, and 9.4 g trehalose 6-phosphate dipotassium salt is obtained Lactococcus lactis subsp. lactis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactococcus lactis subsp. lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucose 6-phosphate + beta-D-glucose 1-phosphate Lactococcus lactis subsp. lactis trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses alpha,alpha-trehalose 6-phosphate + phosphate
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Organism

Organism UniProt Comment Textmining
Lactococcus lactis subsp. lactis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucose 6-phosphate + beta-D-glucose 1-phosphate
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Lactococcus lactis subsp. lactis alpha,alpha-trehalose 6-phosphate + phosphate
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r
glucose 6-phosphate + beta-D-glucose 1-phosphate trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses Lactococcus lactis subsp. lactis alpha,alpha-trehalose 6-phosphate + phosphate
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r

Synonyms

Synonyms Comment Organism
TrePP
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Lactococcus lactis subsp. lactis