Application | Comment | Organism |
---|---|---|
synthesis | efficient one-pot enzymatic synthesis of trehalose 6-phosphate. The one-pot reaction of trehalose 6-phosphate phosphorylase and maltose phosphorylase enables production of 65 mM trehalose 6-phosphate from 100 mM maltose, 100 mM Glc6P, and 20 mM inorganic phosphate. Addition of beta-phosphoglucomutase to this reaction produces glucose 6-phosphate from beta-glucose 1-phosphate and thus reduces requirement of glucose 6-phosphate as a starting material. Within the range of 20-469 mM inorganic phosphate tested, the 54 mM concentration yielded the highest amount of trehalose 6-phosphate (33 mM). Addition of yeast increases the yield because of its glucose consumption. From 100 mmol maltose and 60 mmol inorganic phosphate, the production of 37.5 mmol trehalose 6-phosphate in a one-pot reaction (100 ml) is achieved, and 9.4 g trehalose 6-phosphate dipotassium salt is obtained | Lactococcus lactis subsp. lactis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lactococcus lactis subsp. lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucose 6-phosphate + beta-D-glucose 1-phosphate | Lactococcus lactis subsp. lactis | trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses | alpha,alpha-trehalose 6-phosphate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis subsp. lactis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
Lactococcus lactis subsp. lactis | alpha,alpha-trehalose 6-phosphate + phosphate | - |
r | |
glucose 6-phosphate + beta-D-glucose 1-phosphate | trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses | Lactococcus lactis subsp. lactis | alpha,alpha-trehalose 6-phosphate + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TrePP | - |
Lactococcus lactis subsp. lactis |