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Literature summary for 2.4.1.214 extracted from

  • Both, P.; Sobczak, L.; Breton, C.; Hann, S.; Noebauer, K.; Paschinger, K.; Kozmon, S.; Mucha, J.; Wilson, I.B.
    Distantly related plant and nematode core alpha1,3-fucosyltransferases display similar trends in structure-function relationships (2011), Glycobiology, 21, 1401-1415.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.214

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Caenorhabditis elegans
expressed in Pichia pastoris strain GS115 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D303E the mutant retains 23.8% of the wild type activity Caenorhabditis elegans
N194Q glycosylation site mutant with less than 1% of wild type activity Caenorhabditis elegans
N219A the mutant retains 1.2% of the wild type activity Arabidopsis thaliana
N337A the mutant retains 18.6% of the wild type activity Arabidopsis thaliana
R226A the mutation leads to a complete loss of activity Arabidopsis thaliana
S218A the mutation completely abolishes the enzyme activity Arabidopsis thaliana
S243A the mutant retains 16.2% of the wild type activity Caenorhabditis elegans
S253A the mutant retains 35.6% of the wild type activity Arabidopsis thaliana
S483A the mutant retains 32.2% of the wild type activity Arabidopsis thaliana
T339A the mutant retains 12.1% of the wild type activity Arabidopsis thaliana
T361A the mutant retains 24.3% of the wild type activity Caenorhabditis elegans
T422A the mutant retains 36.5% of the wild type activity Arabidopsis thaliana
Y243A the mutant retains 9.8% of the wild type activity Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 3% residual activity in the presence of 1 mM Arabidopsis thaliana
Cu2+ 3% residual activity in the presence of 1 mM Caenorhabditis elegans
EDTA about 5% residual activity in the presence of 10 mM Arabidopsis thaliana
EDTA about 5% residual activity in the presence of 10 mM Caenorhabditis elegans
Zn2+ 3% residual activity in the presence of 1 mM Arabidopsis thaliana
Zn2+ 3% residual activity in the presence of 1 mM Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 GDP-beta-L-fucose mutant enzyme S253A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
0.24
-
 GDP-beta-L-fucose mutant enzyme Y243A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
0.33
-
 GDP-beta-L-fucose mutant enzyme N219A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
0.36
-
 GDP-beta-L-fucose mutant enzyme T339A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
1
-
 GDP-beta-L-fucose wild type enzyme, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
Ca2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana
Co2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
Co2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana
Mg2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
Mg2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana
Mn2+ highest relative enzyme activity in the presence of Mn2+ Caenorhabditis elegans
Mn2+ highest relative enzyme activity in the presence of Mn2+ Arabidopsis thaliana
additional information Zn2+ and Cu2+ ions do not activate the enzyme Caenorhabditis elegans
additional information Zn2+ and Cu2+ ions do not activate the enzyme Arabidopsis thaliana
Ni2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
Ni2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, calculated from amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1-2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc Arabidopsis thaliana
-
 GDP + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(Fucalpha(1->3))GlcNAc
-
 ?
GDP-beta-L-fucose + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc Caenorhabditis elegans
-
 GDP + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C8W3
-
-
Caenorhabditis elegans G5EDR5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Caenorhabditis elegans
glycoprotein
-
 Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA or HisTrap column chromatography Caenorhabditis elegans
Ni-NTA or HisTrap column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1-2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
 Arabidopsis thaliana GDP + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(Fucalpha(1->3))GlcNAc
-
 ?
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc
-
 Arabidopsis thaliana GDP + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?
GDP-beta-L-fucose + Manalpha(1->6)(Manalpha(1->3))Manalpha(1->6)(Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
 Caenorhabditis elegans GDP + Manalpha(1->6)(Manalpha(1->3))Manalpha(1->6)(Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)[Fucalpha(1->3)]GlcNAc
-
 ?
GDP-beta-L-fucose + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc
-
 Caenorhabditis elegans GDP + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, calculated from amino acid sequence Arabidopsis thaliana
? x * 60000-70000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
core alpha1,3-fucosyltransferase
-
 Caenorhabditis elegans
core alpha1,3-fucosyltransferase
-
 Arabidopsis thaliana
FucTA
-
 Arabidopsis thaliana
FUT-1
-
 Caenorhabditis elegans
FUT11
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
 Caenorhabditis elegans
30
-
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Arabidopsis thaliana
7.5
-
-
 Caenorhabditis elegans