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Literature summary for 2.4.1.214 extracted from

  • Campbell, C.; Stanley, P.
    The Chinese hamster ovary glycosylation mutants LEC11 and LEC12 express two novel GDP-fucose:N-acetylglucosaminide 3-alpha-L-fucosyltransferase enzymes (1984), J. Biol. Chem., 259, 11208-11214.
    View publication on PubMed
Search for more literature for 2.4.1.214

Inhibitors

Inhibitors Comment Organism Structure
GDP inhibits both LEC11 and LEC12 cells enzyme activity to approximately the same extent Cricetulus griseus
GMP inhibits both LEC11 and LEC12 cells enzyme activity to approximately the same extent Cricetulus griseus
N-ethylmaleimide at 1 mM completely inhibits the enzyme activity in LEC11 cells, in LEC12 cells: not inhibitory Cricetulus griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine Cricetulus griseus
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 GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
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 ?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
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-
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Source Tissue

Source Tissue Comment Organism Textmining
ovary glycosylation mutants LEC11 and LEC12 Cricetulus griseus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000001
-
 LEC12 mutant, substrate: alpha1-acid glycoprotein Cricetulus griseus
0.000002
-
 LEC11 mutant with transferrin as substrate and LEC12 mutant with fetuin as substrate Cricetulus griseus
0.000003
-
 LEC12 mutant, substrate: transferrin Cricetulus griseus
0.000007
-
 LEC11 mutant, substrate: asialotransferrin Cricetulus griseus
0.000013
-
 LEC11 mutant, substrate: asialofetuin Cricetulus griseus
0.000014
-
 LEC11 mutant with fetuin as substrate and LEC12 mutant with asialofetuin as substrate Cricetulus griseus
0.000015
-
 LEC11 mutant, substrate: alpha1-acid glycoprotein Cricetulus griseus
0.000016
-
 LEC12 mutant, substrate: asialo-alpha1-acid glycoprotein or asialotransferrin Cricetulus griseus
0.000019
-
 LEC11 mutant, substrate: asialo-alpha1-acid glycoprotein Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + alpha1-acid glycoprotein the activity of the LEC11 cells enzyme is considerably higher than the activity of the LEC12 cells enzyme Cricetulus griseus GDP + alpha1-3 fucosylated glycoprotein
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 ?
GDP-fucose + asialo-alpha1-acid glycoprotein
-
 Cricetulus griseus GDP + alpha1-3 fucosylated glycoprotein
-
 ?
GDP-fucose + asialofetuin
-
 Cricetulus griseus GDP + alpha1-3 fucosylated glycoprotein
-
 ?
GDP-fucose + asialotransferrin
-
 Cricetulus griseus GDP + alpha1-3 fucosylated glycoprotein
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 ?
GDP-fucose + fetuin the activity of the LEC11 cells enzyme is considerably higher than the activity of the LEC12 cells enzyme Cricetulus griseus GDP + alpha1-3 fucosylated glycoprotein
-
 ?
GDP-fucose + transferrin
-
 Cricetulus griseus GDP + alpha1-3 fucosylated glycoprotein
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Cricetulus griseus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?