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Literature summary for 2.4.1.212 extracted from

  • Siiskonen, H.; Kaernae, R.; Hyttinen, J.M.; Tammi, R.H.; Tammi, M.I.; Rilla, K.
    Hyaluronan synthase 1 (HAS1) produces a cytokine-and glucose-inducible, CD44-dependent cell surface coat (2014), Exp. Cell Res., 320, 153-163.
    View publication on PubMed
Search for more literature for 2.4.1.212

Cloned(Commentary)

Cloned (Comment) Organism
gene HAS1, recombinant expression of GFP-tagged isozyme HAS1 in MCF-7 cells, MCF-7 cells transfected with Dendra-Has1 show intense fluorescence in perinuclear vesicular structures resembling the Golgi apparatus. The MCF-7 cells transfected with EGFP-Has1 produce a pericellular hyaluronan coat, which is attached the synthase itself Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information HAS1-transfected MCF-7cells show very little cell surface hyaluronan, but a large hyaluronan coat is seen in cells grown in 20 mM glucose and 1 mM glucosamine, or treated with interleukin-1beta, TNF-alpha, or TGF-beta. The coats are mostly removed by the presence of hyaluronan hexasaccharides, or Hermes1 antibody, indicating that they depend on the CD44 receptor, which is, in contrast to the coat produced by HAS3, remaining attached to HAS3 itself Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-Methylumbelliferone
-
 Homo sapiens
brefeldin A
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Homo sapiens 9986
-
Golgi apparatus
-
 Homo sapiens 5794
-
additional information the HAS1 enzymatic activity depends on the endoplasmic reticulum-Golgi-plasma membrane traffic, like reported for isozymes HAS2 and HAS3 Homo sapiens
-
-
plasma membrane
-
 Homo sapiens 5886
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 gene HAS1
-

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
 Homo sapiens
-
epidermis
-
 Homo sapiens
-
HaCaT cell
-
 Homo sapiens
-
keratinocyte
-
 Homo sapiens
-
MCF-7 cell very low expression of HAS1 Homo sapiens
-
additional information subcellular localization study, intracellular localization of EGFP-HAS1 and Dendra-HAS1 in MCF-7 cells, overview Homo sapiens
-

Synonyms

Synonyms Comment Organism
HAS1
-
 Homo sapiens
hyaluronan synthase 1
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens a cytokine-and glucose-inducible enzyme up

General Information

General Information Comment Organism
physiological function hyaluronan is a ubiquitous glycosaminoglycan involved in embryonic development, inflammation and cancer. In mammals, three hyaluronan synthase isoenzymes (HAS1-3) inserted in the plasma membrane produce hyaluronan directly on the cell surface. Isozyme hyaluronan synthase 1 (HAS1) produces a cytokine-and glucose-inducible, CD44-dependent cell surface coat Homo sapiens