Cloned (Comment) | Organism |
---|---|
expression of C-terminally His6-tagged Se-HAS in Escherichia coli strain C43 | Streptococcus dysgalactiae subsp. equisimilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | purified Se-HAS is reconstituted into proteoliposomes, from total lipid extract from Escherichia coli, where it synthesizes and translocates hyaluronan, or reconstituted into synthetic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine/1,1',2,2'-tetraoleoyl cardiolipin/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (80/10/10%) proteoliposomes. In vitro synthesized, high-molecular-weight hyaluronan remains tightly associated with the intact proteoliposomes, even after proteolyticdegradation of HAS or in the presence of 1 M NaCl or 0.6 M urea to prevent nonspecific interactions of the polymer with the lipid vesicles | Streptococcus dysgalactiae subsp. equisimilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | the hyaluronan polymer is synthesized on the cytosolic side of the cell membrane by the membrane-embedded hyaluronan synthase | Streptococcus dysgalactiae subsp. equisimilis | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus dysgalactiae subsp. equisimilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia ccoli strain C43 by solubilization with detergent LysoFosCholine Ether-14, nickel affinity chromatography, and gel filtration | Streptococcus dysgalactiae subsp. equisimilis |
Synonyms | Comment | Organism |
---|---|---|
HAS | - |
Streptococcus dysgalactiae subsp. equisimilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptococcus dysgalactiae subsp. equisimilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptococcus dysgalactiae subsp. equisimilis |
General Information | Comment | Organism |
---|---|---|
physiological function | the hyaluronan synthase catalyzes the synthesis and membrane translocation of hyaluronan, it is both necessary and sufficient to translocate hyaluronan in a reaction that is tightly coupled to hyaluronan elongation. Hyaluronan synthesis and translocation are spatially coupled events, which allow hyaluronan synthesis even in the presence of a large excess of hyaluronan-degrading enzyme | Streptococcus dysgalactiae subsp. equisimilis |