Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli | Streptococcus dysgalactiae subsp. equisimilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Streptococcus dysgalactiae subsp. equisimilis | |
Mg2+ | - |
Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | Streptococcus dysgalactiae subsp. equisimilis | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | Streptococcus pyogenes | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus dysgalactiae subsp. equisimilis | - |
class I enzyme | - |
Streptococcus pyogenes | - |
class I enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Streptococcus dysgalactiae subsp. equisimilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | Streptococcus dysgalactiae subsp. equisimilis | |
UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | determination of polymer synthesis progression direction, overview | Streptococcus dysgalactiae subsp. equisimilis | ? | - |
? | |
additional information | determination of polymer synthesis progression direction, overview | Streptococcus pyogenes | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | Streptococcus dysgalactiae subsp. equisimilis | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | Streptococcus pyogenes | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | Streptococcus dysgalactiae subsp. equisimilis | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | product chain length depends on reaction conditions, overview | ? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | addition of monosaccharides to the reducing end to form a linear heteropolysaccharide chain | Streptococcus pyogenes | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | product chain length depends on reaction conditions, overview | ? |
Synonyms | Comment | Organism |
---|---|---|
HASs | - |
Streptococcus dysgalactiae subsp. equisimilis |
HASs | - |
Streptococcus pyogenes |
More | enzyme belongs to class I of hyaluronan synthases | Streptococcus dysgalactiae subsp. equisimilis |
More | enzyme belongs to class I of hyaluronan synthases | Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Streptococcus dysgalactiae subsp. equisimilis |
30 | - |
assay at | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptococcus dysgalactiae subsp. equisimilis |
7.5 | - |
assay at | Streptococcus pyogenes |