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Literature summary for 2.4.1.211 extracted from

  • Nakajima, M.; Nishimoto, M.; Kitaoka, M.
    Characterization of beta-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes (2009), Appl. Microbiol. Biotechnol., 83, 109-115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cutibacterium acnes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
N-acetyl-D-galactosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
130
-
N-acetyl-D-glucosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
140
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
1300
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
His-tagged enzyme, SDS-PAGE Cutibacterium acnes
83480
-
His-tagged enzyme, calculated from amino acid sequence Cutibacterium acnes

Organism

Organism UniProt Comment Textmining
Cutibacterium acnes B7XEJ0 strain JCM6473
-
Cutibacterium acnes B7XEJ1 strain JCM6425
-
Cutibacterium acnes JCM 6425 B7XEJ1 strain JCM6425
-
Cutibacterium acnes JCM 6473 B7XEJ0 strain JCM6473
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Cutibacterium acnes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
-
Cutibacterium acnes alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine Cutibacterium acnes alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine specific activity on N-acetyl-D-galactosamine is approximately 60times higher than that on N-acetyl-D-glucosamine r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine Cutibacterium acnes JCM 6425 alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine specific activity on N-acetyl-D-galactosamine is approximately 60times higher than that on N-acetyl-D-glucosamine r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
-
Cutibacterium acnes JCM 6473 alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Cutibacterium acnes alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine Cutibacterium acnes alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine Cutibacterium acnes JCM 6425 alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Cutibacterium acnes JCM 6473 alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?
additional information GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate Cutibacterium acnes ?
-
?
additional information GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate Cutibacterium acnes JCM 6425 ?
-
?
additional information GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate Cutibacterium acnes JCM 6473 ?
-
?

Synonyms

Synonyms Comment Organism
beta-1,3-galactosyl-N-acetylhexosamine phosphorylase
-
Cutibacterium acnes
galacto-N-biose phosphorylase
-
Cutibacterium acnes
GalHexNAcP
-
Cutibacterium acnes
GnpA
-
Cutibacterium acnes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Cutibacterium acnes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the enzyme is stable up to 37°C Cutibacterium acnes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.9
-
N-acetyl-D-glucosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
14
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
42
-
N-acetyl-D-galactosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
103
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Cutibacterium acnes

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5
-
Cutibacterium acnes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
0.044
-
N-acetyl-D-glucosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
0.73
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes
3.9
-
N-acetyl-D-galactosamine in 100 mM MOPS buffer (pH 7.0) at 37°C Cutibacterium acnes